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Literature summary for 1.14.19.17 extracted from

  • Michel, C.; Van Echten-Deckert, G.; Rother, J.; Sandhoff, K.; Wang, E.; Merrill Jr., A.
    Characterization of ceramide synthesis. A dihydroceramide desaturase introduces the 4,5-trans-double bond of sphingosine at the level of dihydroceramide (1997), J. Biol. Chem., 272, 22432-22437.
    View publication on PubMed
Search for more literature for 1.14.19.17

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid inhibitory at high concentrations Rattus norvegicus
Cu2+ 25% inhibition at 1 mM Rattus norvegicus
cyanide
-
 Rattus norvegicus
dithiothreitol 81% inhibition at 1 mM Rattus norvegicus
additional information not inhibited by EDTA and Na2SO4 Rattus norvegicus
N-acetylcysteine
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 NADH with N-octanoyl-D-erythro-C18-sphinganine as cosubstrate, at pH 7.4 and 37°C Rattus norvegicus
0.34
-
 N-octanoyl-D-erythro-C18-sphinganine at pH 7.4 and 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by Ca2+, Mg2+, Mn2+, Fe2+, K+, and Na+ Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q564G3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hepatocyte
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-4°C, phosphate buffer (100 mM NaH2PO4/Na2HPO4, pH 7.4), 7 days, approximately 50% of the activity is lost Rattus norvegicus
-80°C, phosphate buffer (100 mM NaH2PO4/Na2HPO4, pH 7.4), 1 year, approximately 50% of the activity is lost Rattus norvegicus
37°C, phosphate buffer (100 mM NaH2PO4/Na2HPO4, pH 7.4), 3 h, approximately 50% of the activity is lost Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrosphingomyelin + NADH + O2 + H+ 20% activity compared to N-octanoyl-D-erythro-C18-sphinganine Rattus norvegicus ?
-
 ?
additional information no activity detected with dihydroglucosylceramide Rattus norvegicus ?
-
 ?
N-octanoyl-D-erythro-C18-sphinganine + NADH + O2 + H+
-
 Rattus norvegicus N-octanoyl-D-erythro-C18-sphingosine + NAD+ + H2O
-
 ?
N-octanoyl-D-erythro-C18-sphinganine + NADPH + O2 + H+
-
 Rattus norvegicus N-octanoyl-D-erythro-C18-sphingosine + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
dihydroceramide desaturase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43
-
-
 Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
27 47 the half-maximal enzymatic activities are at 27°C and 47°C. Above 60°C, no activity is detected Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
cytochrome b5
-
 Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.031
-
 at pH 7.4 and 37°C Rattus norvegicus Cu2+
0.1
-
 at pH 7.4 and 37°C Rattus norvegicus cyanide