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Literature summary for 1.14.19.13 extracted from

  • Bucek, A.; Matouskova, P.; Sychrova, H.; Pichova, I.; Hruskova-Heidingsfeldova, O.
    DELTA12-Fatty acid desaturase from Candida parapsilosis is a multifunctional desaturase producing a range of polyunsaturated and hydroxylated fatty acids (2014), PLoS ONE, 9, e93322 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.19.13

Cloned(Commentary)

Cloned (Comment) Organism
gene FAD2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression in Saccharomyces cerevisiae strain BY4741 supplemented with linoleic acid (DELTA9,DELTA12-18:2) and hexadecadienoic acid (DELTA9,DELTA12-16:2) leads to accumulation of DELTA15-PUFAs, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3) results in production of a range of DELTA12- and DELTA15-PUFAs. The major products of CpFad2 are linoleic and hexadecadienoic acid (DELTA9,DELTA12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (DELTA9,DELTA12,DELTA15-16:3). Ricinoleic acid (12-hydroxy-9-octadecenoic acid) is an additional product of CpFad2 identified by GC/MS analysis Candida parapsilosis
gene FAD3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression in Saccharomyces cerevisiae strain BY4741 supplemented with linoleic acid (DELTA9,DELTA12-18:2) and hexadecadienoic acid (DELTA9,DELTA12-16:2) leads to accumulation of DELTA15-PUFAs, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3) results in production of DELTA15-polyunsaturated fatty acids, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3), identified by GC/MS analysis Candida parapsilosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Candida parapsilosis analysis of content in polyunsaturated and hydroxylated fatty acids in the cells, mass spectrometric analysis, overview ?
-
 ?
additional information Candida parapsilosis CP-69 analysis of content in polyunsaturated and hydroxylated fatty acids in the cells, mass spectrometric analysis, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Candida parapsilosis C3W956
-
-
Candida parapsilosis C3W957
-
-
Candida parapsilosis CP-69 C3W956
-
-
Candida parapsilosis CP-69 C3W957
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information analysis of content in polyunsaturated and hydroxylated fatty acids in the cells, mass spectrometric analysis, overview Candida parapsilosis ?
-
 ?
additional information recombinant CpFad3 produces DELTA15-polyunsaturated fatty acids, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3), identified by GC/MS analysis Candida parapsilosis ?
-
 ?
additional information unambiguous identification of the triunsaturated product of CpFad2 as DELTA9,DELTA12,DELTA15-16:3 with terminal (n-1) double bond by comparing its spectrum to that of DELTA9,DELTA12,DELTA15-16:3 methylester. The major products of recombinant CpFad2 are linoleic and hexadecadienoic acid (DELTA9,DELTA12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (DELTA9,DELTA12,DELTA15-16:3). Ricinoleic acid (12-hydroxy-9-octadecenoic acid) is an additional product of CpFad2 identified by GC/MS analysis Candida parapsilosis ?
-
 ?
additional information analysis of content in polyunsaturated and hydroxylated fatty acids in the cells, mass spectrometric analysis, overview Candida parapsilosis CP-69 ?
-
 ?
additional information unambiguous identification of the triunsaturated product of CpFad2 as DELTA9,DELTA12,DELTA15-16:3 with terminal (n-1) double bond by comparing its spectrum to that of DELTA9,DELTA12,DELTA15-16:3 methylester. The major products of recombinant CpFad2 are linoleic and hexadecadienoic acid (DELTA9,DELTA12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (DELTA9,DELTA12,DELTA15-16:3). Ricinoleic acid (12-hydroxy-9-octadecenoic acid) is an additional product of CpFad2 identified by GC/MS analysis Candida parapsilosis CP-69 ?
-
 ?
additional information recombinant CpFad3 produces DELTA15-polyunsaturated fatty acids, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3), identified by GC/MS analysis Candida parapsilosis CP-69 ?
-
 ?

Synonyms

Synonyms Comment Organism
CpFAD2
-
 Candida parapsilosis
CpFAD3
-
 Candida parapsilosis
DELTA12-fatty acid desaturase
-
 Candida parapsilosis
DELTA15-fatty acid desaturase
-
 Candida parapsilosis
More cf. EC 1.14.19.45 Candida parapsilosis

General Information

General Information Comment Organism
physiological function DELTA12-fatty acid desaturase CpFAD2 from Candida parapsilosis is a multifunctional desaturase producing a range of DELTA12- and DELTA15-polyunsaturated and hydroxylated fatty acids Candida parapsilosis
physiological function DELTA12-fatty acid desaturase CpFAD3 from Candida parapsilosis is a DELTA15-desaturase producing DELTA15-polyunsaturated fatty acids, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3), overview Candida parapsilosis