Cloned (Comment) | Organism |
---|---|
gene RKD12, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae | Rhodotorula kratochvilovae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rhodotorula kratochvilovae | the enzyme produces linoleic acid and alpha-linolenic acid, overview | ? | - |
? | |
additional information | Rhodotorula kratochvilovae YM25235 | the enzyme produces linoleic acid and alpha-linolenic acid, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula kratochvilovae | A0A024B710 | isolated from Chenghai Lake in Yunnan, China | - |
Rhodotorula kratochvilovae YM25235 | A0A024B710 | isolated from Chenghai Lake in Yunnan, China | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA + reduced acceptor + O2 | - |
Rhodotorula kratochvilovae | (9Z,12Z,15Z)-hexadeca-9,12,15-trienoyl-CoA + acceptor + 2 H2O | - |
? | |
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA + reduced acceptor + O2 | - |
Rhodotorula kratochvilovae YM25235 | (9Z,12Z,15Z)-hexadeca-9,12,15-trienoyl-CoA + acceptor + 2 H2O | - |
? | |
additional information | the enzyme produces linoleic acid and alpha-linolenic acid, overview | Rhodotorula kratochvilovae | ? | - |
? | |
additional information | absence of desaturation activity of RKD12 for C16 substrates | Rhodotorula kratochvilovae | ? | - |
? | |
additional information | the enzyme produces linoleic acid and alpha-linolenic acid, overview | Rhodotorula kratochvilovae YM25235 | ? | - |
? | |
additional information | absence of desaturation activity of RKD12 for C16 substrates | Rhodotorula kratochvilovae YM25235 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50600, about, sequence calculation | Rhodotorula kratochvilovae |
Synonyms | Comment | Organism |
---|---|---|
bifunctional DELTA12/DELTA15-fatty acid desaturase | - |
Rhodotorula kratochvilovae |
DELTA12-desaturase | - |
Rhodotorula kratochvilovae |
More | cf. EC 1.14.19.45 | Rhodotorula kratochvilovae |
RKD12 | - |
Rhodotorula kratochvilovae |
Organism | Comment | Expression |
---|---|---|
Rhodotorula kratochvilovae | a culture temperature downshift from 28°C to 15°C induces the enzyme | up |
General Information | Comment | Organism |
---|---|---|
evolution | bifunctional DELTA12/DELTA15-desaturases are widely found in fungi species belonging to Ascomycota and Basidiomycota. Enzyme RKD12 shares higher homology to the Cop-odeA, it only exhibits DELTA12 and DELTA15 desaturation activity on C18 substrates without preference | Rhodotorula kratochvilovae |
physiological function | for better adaptation to low temperatures, Rhodosporidium kratochvilovae YM25235 may have evolved some adaptative mechanisms that might include the alteration of unsaturation levels of fatty acids in membrane lipids involving the enzyme | Rhodotorula kratochvilovae |