Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | Chlamydomonas reinhardtii | DELTA5-desaturase activity | coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O | - |
? | |
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | Chlamydomonas reinhardtii 137C | DELTA5-desaturase activity | coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O | - |
? | |
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | Chlamydomonas reinhardtii | low activity | ? | - |
? | |
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | Chlamydomonas reinhardtii 137C | low activity | ? | - |
? | |
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | Chlamydomonas reinhardtii | low activity | ? | - |
? | |
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | Chlamydomonas reinhardtii 137C | low activity | ? | - |
? | |
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | Chlamydomonas reinhardtii | preferred substrate, DELTA5-desaturase activity | pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O | - |
? | |
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | Chlamydomonas reinhardtii 137C | preferred substrate, DELTA5-desaturase activity | pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O | - |
? | |
additional information | Chlamydomonas reinhardtii | no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively | ? | - |
? | |
additional information | Chlamydomonas reinhardtii 137C | no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively | ? | - |
? | |
oleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | Chlamydomonas reinhardtii | low activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | Q2HWK7 | - |
- |
Chlamydomonas reinhardtii 137C | Q2HWK7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | DELTA5-desaturase activity | Chlamydomonas reinhardtii | coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O | - |
? | |
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | DELTA5-desaturase activity | Chlamydomonas reinhardtii 137C | coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O | - |
? | |
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | low activity | Chlamydomonas reinhardtii | ? | - |
? | |
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | low activity | Chlamydomonas reinhardtii 137C | ? | - |
? | |
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | low activity | Chlamydomonas reinhardtii | ? | - |
? | |
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | low activity | Chlamydomonas reinhardtii 137C | ? | - |
? | |
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | preferred substrate, DELTA5-desaturase activity | Chlamydomonas reinhardtii | pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O | - |
? | |
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | preferred substrate, DELTA5-desaturase activity | Chlamydomonas reinhardtii 137C | pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O | - |
? | |
additional information | no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively | Chlamydomonas reinhardtii | ? | - |
? | |
additional information | no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively | Chlamydomonas reinhardtii 137C | ? | - |
? | |
oleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ | low activity | Chlamydomonas reinhardtii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DES | - |
Chlamydomonas reinhardtii |
omega13 desaturase | - |
Chlamydomonas reinhardtii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Chlamydomonas reinhardtii |