Literature summary for 1.14.19.1 extracted from

Kachroo, P.; Shanklin, J.; Shah, J.; Whittle, E.J.; Klessig, D.F.
A fatty acid desaturase modulates the activation of defense signaling pathways in plants (2001), Proc. Natl. Acad. Sci. USA, 98, 9448-9453.

Search for more literature for 1.14.19.1

Application

Application	Comment	Organism
agriculture	disease resistance	Arabidopsis sp.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, transfection of Arabidopsis by Agrobacterium tumefaciens clones	Arabidopsis sp.

Protein Variants

Protein Variants	Comment	Organism
L146F	point mutation in ssi2 gene, causes reduced desaturase activity	Arabidopsis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
stearoyl-acyl-carrier protein + AH2 + O2	Arabidopsis sp.	key regulator of fatty acid desaturation of carbons 9 and 10	oleoyl-acyl-carrier protein + A + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Arabidopsis sp.

Reaction

Reaction	Comment	Organism	Reaction ID
stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O	SSI2 gene encodes a member of a family of soluble fatty acid desaturases	Arabidopsis sp.	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis sp.	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.8	-	-	Arabidopsis sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	preference for 18-carbon versus 16-carbon chain length fatty acids	Arabidopsis sp.	?	-	?
stearoyl-acyl-carrier protein + AH2 + O2	-	Arabidopsis sp.	oleoyl-acyl-carrier protein + A + H2O	-	?
stearoyl-acyl-carrier protein + AH2 + O2	key regulator of fatty acid desaturation of carbons 9 and 10	Arabidopsis sp.	oleoyl-acyl-carrier protein + A + H2O	-	?
stearoyl-CoA + AH2 + O2	-	Arabidopsis sp.	oleoyl-CoA + A + H2O	-	?

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Release 2023.1 (January 2023)