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Literature summary for 1.14.19.1 extracted from
- A function of cytochrome b5 in fatty acid desaturation by rat liver microsomes (1971), J. Biochem., 69, 155-167.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b5 reductase | NADH-specific, required, EC 1.6.2.2 | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | - |
Rattus norvegicus |  |
| CN- | - |
Rattus norvegicus | |
| HgCl2 | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| stearoyl-CoA + AH2 + O2 | Rattus norvegicus | - |
oleoyl-CoA + A + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Sprague-Dawley, male | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O | the reducing equivalents required for the desaturation site seem to be supplied from NADPH via cytochrome b5 as electron carrier | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose | epididymal | Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0023 | - |
fed microsomes | Rattus norvegicus |
| 0.0079 | - |
induced microsomes | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| stearoyl-CoA + AH2 + O2 | - |
Rattus norvegicus | oleoyl-CoA + A + H2O | - |
? | |
| stearoyl-CoA + NAD(P)H + O2 | fatty acid desaturation | Rattus norvegicus | oleoyl-CoA + NAD(P)+ + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
with NADH and NADPH | Rattus norvegicus |
| 7.2 | - |
assay at | Rattus norvegicus |
| 7.8 | - |
with ascorbate | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | - |
Rattus norvegicus | |
| cytochrome b5 | required | Rattus norvegicus | |
| NADH | - |
Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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