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Literature summary for 1.14.18.9 extracted from
- Affinity chromatography of microsomal enzymes on immobilized detergent-solubilized cytochrome b5 (1986), J. Biol. Chem., 261, 3790-3799 .
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| polyethylene glycol fractionation, DEAE-Sephacel gel filtration, CM-Sepharose column chromatography, and cytochrome b5-Sepharose column chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.5 | - |
crude extract, at pH 7.4 and °C | Rattus norvegicus |
| 17.2 | - |
after 34fold purification, at pH 7.4 and °C | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4alpha-methyl-5alpha-cholest-7-en-3beta-ol + ferrocytochrome b5 + O2 + H+ | - |
Rattus norvegicus | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-methyl sterol oxidase | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b5 | dependent on | Rattus norvegicus |
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Release 2023.1 (January 2023)
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