BRENDA - Enzyme Database
show all sequences of 1.14.18.8

Assays for cholesterol 7alpha-hydroxylase and 12alpha-hydroxylase using high performance liquid chromatography

Noshiro, M.; Ishida, H.; Hayashi, S.; Okuda, K.; Steroids 45, 539-550 (1985)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000885
-
liver microsome
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
7alpha-hydroxycholest-4-en-3-one + NADH + H+ + O2
reaction with NADH shows 15% of the activity with NADPH
660512
Rattus norvegicus
7alpha,12alpha-dihydroxycholest-4-en-3-one + NAD+ + H2O
-
-
-
?
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2
NADPH is the preferred electron source
660512
Rattus norvegicus
7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
HCO 12alpha-hydroxylase
-
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
15% of the activity with NADPH
Rattus norvegicus
NADPH
preferred electron source
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
15% of the activity with NADPH
Rattus norvegicus
NADPH
preferred electron source
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000885
-
liver microsome
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
7alpha-hydroxycholest-4-en-3-one + NADH + H+ + O2
reaction with NADH shows 15% of the activity with NADPH
660512
Rattus norvegicus
7alpha,12alpha-dihydroxycholest-4-en-3-one + NAD+ + H2O
-
-
-
?
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2
NADPH is the preferred electron source
660512
Rattus norvegicus
7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
-
-
?
Other publictions for EC 1.14.18.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
688997
Lee
Liver receptor homolog-1 regul ...
Mus musculus
Mol. Endocrinol.
22
1345-1356
2008
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-
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-
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-
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3
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1
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1
-
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1
-
-
700579
Liu
Dexamethasone exposure of neon ...
Rattus norvegicus
Pediatr. Res.
63
375-381
2008
-
-
-
-
-
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-
-
-
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4
-
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-
1
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1
-
-
658153
Andersson
Rabbit liver contains one majo ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
1389
150-154
1998
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-
1
-
-
-
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1
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4
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1
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2
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1
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1
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1
1
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1
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1
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2
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1
-
-
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-
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-
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-
-
659131
Eggertsen
Molecular cloning and expressi ...
Oryctolagus cuniculus, Mus musculus, Rattus norvegicus
J. Biol. Chem.
271
32269-32275
1996
-
-
1
-
-
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-
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8
-
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-
1
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3
-
4
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3
-
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1
3
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1
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3
-
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-
-
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-
659123
Ishida
Purification and characterizat ...
Oryctolagus cuniculus
J. Biol. Chem.
267
21319-21323
1992
1
-
-
-
-
-
-
-
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1
1
-
1
-
1
1
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1
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2
1
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3
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1
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3
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1
1
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1
1
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1
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2
1
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658237
Kimura
Effect of streptozotocin-induc ...
Rattus norvegicus
Biol. Chem. Hoppe-Seyler
369
1117-1120
1988
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-
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1
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1
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1
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1
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2
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1
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1
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1
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1
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1
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2
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-
660512
Noshiro
Assays for cholesterol 7alpha- ...
Rattus norvegicus
Steroids
45
539-550
1985
-
-
-
-
-
-
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1
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1
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1
1
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2
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1
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2
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2
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1
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1
1
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2
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659116
Murakami
Purification and characterizat ...
Oryctolagus cuniculus
J. Biol. Chem.
257
8030-8035
1982
1
-
-
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-
1
4
1
1
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1
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1
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1
-
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1
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1
-
1
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1
1
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2
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1
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2
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1
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4
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1
1
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1
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1
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1
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1
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1
1
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