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Literature summary for 1.14.18.3 extracted from

  • Murrell, J.C.; Gilbert, B.; McDonald, I.R.
    Molecular biology and regulation of methane monooxygenase (2000), Arch. Microbiol., 173, 325-332.
    View publication on PubMed
Search for more literature for 1.14.18.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Methylococcus capsulatus
expressed in Escherichia coli Methylomicrobium album
expressed in Escherichia coli Methylocystis sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Methylococcus capsulatus 16020
-
membrane
-
 Methylomicrobium album 16020
-
membrane
-
 Methylocystis sp. 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the active enzyme contains approximately 15 copper atoms per mol Methylococcus capsulatus
Cu2+ the active enzyme contains approximately 15 copper atoms per mol Methylocystis sp.
Cu2+ the active enzyme contains approximately 15 copper atoms per mol enzyme Methylomicrobium album
Fe2+ the active enzyme contains 2 iron atoms per mol Methylococcus capsulatus
Fe2+ the active enzyme contains 2 iron atoms per mol Methylocystis sp.
Fe2+ the active enzyme contains 2 iron atoms per mol enzyme Methylomicrobium album

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylococcus capsulatus
23000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylomicrobium album
23000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylocystis sp.
27000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylococcus capsulatus
27000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylomicrobium album
27000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylocystis sp.
45000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylococcus capsulatus
45000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylomicrobium album
45000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methane + duroquinol + O2 Methylococcus capsulatus
-
 methanol + duroquinone + H2O
-
 ?
methane + duroquinol + O2 Methylomicrobium album
-
 methanol + duroquinone + H2O
-
 ?
methane + duroquinol + O2 Methylocystis sp.
-
 methanol + duroquinone + H2O
-
 ?
methane + duroquinol + O2 Methylococcus capsulatus Bath
-
 methanol + duroquinone + H2O
-
 ?
methane + duroquinol + O2 Methylomicrobium album BG8
-
 methanol + duroquinone + H2O
-
 ?
methane + reduced acceptor + H* + O2 Methylococcus capsulatus
-
 methanol + acceptor + H2O
-
 ?
methane + reduced acceptor + H* + O2 Methylomicrobium album
-
 methanol + acceptor + H2O
-
 ?
methane + reduced acceptor + H* + O2 Methylocystis sp.
-
 methanol + acceptor + H2O
-
 ?
additional information Methylococcus capsulatus unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds ?
-
 ?
additional information Methylomicrobium album unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds ?
-
 ?
additional information Methylocystis sp. unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds ?
-
 ?
additional information Methylococcus capsulatus Bath unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds ?
-
 ?
additional information Methylomicrobium album BG8 unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
-
-
Methylococcus capsulatus Bath
-
-
-
Methylocystis sp.
-
-
-
Methylomicrobium album
-
-
-
Methylomicrobium album BG8
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + duroquinol + O2
-
 Methylococcus capsulatus methanol + duroquinone + H2O
-
 ?
methane + duroquinol + O2
-
 Methylomicrobium album methanol + duroquinone + H2O
-
 ?
methane + duroquinol + O2
-
 Methylocystis sp. methanol + duroquinone + H2O
-
 ?
methane + duroquinol + O2
-
 Methylococcus capsulatus Bath methanol + duroquinone + H2O
-
 ?
methane + duroquinol + O2
-
 Methylomicrobium album BG8 methanol + duroquinone + H2O
-
 ?
methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus methanol + acceptor + H2O
-
 ?
methane + reduced acceptor + H* + O2
-
 Methylomicrobium album methanol + acceptor + H2O
-
 ?
methane + reduced acceptor + H* + O2
-
 Methylocystis sp. methanol + acceptor + H2O
-
 ?
additional information unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds Methylococcus capsulatus ?
-
 ?
additional information unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds Methylomicrobium album ?
-
 ?
additional information unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds Methylocystis sp. ?
-
 ?
additional information unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds Methylococcus capsulatus Bath ?
-
 ?
additional information unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds Methylomicrobium album BG8 ?
-
 ?

Subunits

Subunits Comment Organism
heterotrimer 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylococcus capsulatus
heterotrimer 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylomicrobium album
heterotrimer 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylocystis sp.

Synonyms

Synonyms Comment Organism
particulate methane monooxygenase
-
 Methylococcus capsulatus
particulate methane monooxygenase
-
 Methylomicrobium album
particulate methane monooxygenase
-
 Methylocystis sp.
pMMO
-
 Methylococcus capsulatus
pMMO
-
 Methylomicrobium album
pMMO
-
 Methylocystis sp.

Expression

Organism Comment Expression
Methylococcus capsulatus the pMMO enzyme is expressed in cells grown under high copper-to-biomass ratios up
Methylomicrobium album the pMMO enzyme is expressed in cells grown under high copper-to-biomass ratios up
Methylocystis sp. the pMMO enzyme is expressed in cells grown under high copper-to-biomass ratios up