BRENDA - Enzyme Database
show all sequences of 1.14.18.11

Dissecting the sterol C-4 demethylation process in higher plants. From structures and genes to catalytic mechanism

Rahier, A.; Steroids 76, 340-352 (2011) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Nicotiana benthamiana
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
stigmastan-3beta,5alpha,6alpha-triol
-
Arabidopsis thaliana
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-4-methyl-9,19-cycloergosta-7,24(28)-dien-3-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
-
752272
Arabidopsis thaliana
(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-3-hydroxy-9,19-cycloergosta-7,24(28)-diene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O
-
-
-
ir
(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-4-methylcholest-7-en-3-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
-
752272
Arabidopsis thaliana
(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-3-hydroxycholest-7-ene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O
-
-
-
ir
24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
-
752272
Arabidopsis thaliana
3beta,4alpha,5alpha-3-hydroxystigmasta-7,24(28)-diene-4-carboxylate + 6 ferricytochrome b5 + 4 H2O
-
-
-
?
24-methylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
-
752272
Arabidopsis thaliana
3beta-hydroxyergosta-7,24(241)-dien-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O
-
-
-
ir
Synonyms
Synonyms
Commentary
Organism
4alpha-methyl-DELTA7-sterol-4alpha-methyl-oxidase
-
Arabidopsis thaliana
4alpha-methylsterol-4alpha-methyl-oxidase
-
Arabidopsis thaliana
SMO2
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Nicotiana benthamiana
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
stigmastan-3beta,5alpha,6alpha-triol
-
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-4-methyl-9,19-cycloergosta-7,24(28)-dien-3-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
-
752272
Arabidopsis thaliana
(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-3-hydroxy-9,19-cycloergosta-7,24(28)-diene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O
-
-
-
ir
(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-4-methylcholest-7-en-3-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
-
752272
Arabidopsis thaliana
(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-3-hydroxycholest-7-ene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O
-
-
-
ir
24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
-
752272
Arabidopsis thaliana
3beta,4alpha,5alpha-3-hydroxystigmasta-7,24(28)-diene-4-carboxylate + 6 ferricytochrome b5 + 4 H2O
-
-
-
?
24-methylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
-
752272
Arabidopsis thaliana
3beta-hydroxyergosta-7,24(241)-dien-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O
-
-
-
ir
General Information
General Information
Commentary
Organism
malfunction
when silencing the enzyme, an important accumulation of 4alpha-methyl-DELTA7-sterols is found, with no change in the levels of 4,4-dimethylsterols
Arabidopsis thaliana
metabolism
the enzyme is involved in the removal of the second C4-methyl group of phytosterol precursors
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
malfunction
when silencing the enzyme, an important accumulation of 4alpha-methyl-DELTA7-sterols is found, with no change in the levels of 4,4-dimethylsterols
Arabidopsis thaliana
metabolism
the enzyme is involved in the removal of the second C4-methyl group of phytosterol precursors
Arabidopsis thaliana
Other publictions for EC 1.14.18.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752271
Ramadan
Control of beta-sitosterol bi ...
Calotropis procera
Steroids
141
1-8
2019
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750668
Zhang
De novo sequencing and transc ...
Periploca sepium
Front. Plant Sci.
8
594
2017
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752272
Rahier
Dissecting the sterol C-4 dem ...
Arabidopsis thaliana
Steroids
76
340-352
2011
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657838
Darnet
Plant sterol biosynthesis: ide ...
Nicotiana benthamiana, Nicotiana tabacum, Arabidopsis thaliana
Biochem. J.
378
889-898
2004
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750534
Darnet
Functional identification of ...
Arabidopsis thaliana
FEBS Lett.
508
39-43
2001
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749794
Rahier
The role of cytochrome b5, in ...
Zea mays
Biochem. Biophys. Res. Commun.
236
434-437
1997
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751005
Pascal
Plant sterol biosynthesis. Id ...
Zea mays
J. Biol. Chem.
268
11639-11654
1993
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6
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