Dissecting the sterol C-4 demethylation process in higher plants. From structures and genes to catalytic mechanism

Rahier, A.; Steroids 76, 340-352 (2011)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

stigmastan-3beta,5alpha,6alpha-triol

-

Arabidopsis thaliana

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-4-methyl-9,19-cycloergosta-7,24(28)-dien-3-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+

-

752272

Arabidopsis thaliana

(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-3-hydroxy-9,19-cycloergosta-7,24(28)-diene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O

-

-

-

ir

(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-4-methylcholest-7-en-3-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+

-

752272

Arabidopsis thaliana

(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-3-hydroxycholest-7-ene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O

-

-

-

ir

24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+

-

752272

Arabidopsis thaliana

3beta,4alpha,5alpha-3-hydroxystigmasta-7,24(28)-diene-4-carboxylate + 6 ferricytochrome b5 + 4 H2O

-

-

-

?

24-methylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+

-

752272

Arabidopsis thaliana

3beta-hydroxyergosta-7,24(241)-dien-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O

-

-

-

ir

Inhibitors

Commentary

Organism

Structure

stigmastan-3beta,5alpha,6alpha-triol

-

Arabidopsis thaliana

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-4-methyl-9,19-cycloergosta-7,24(28)-dien-3-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+

-

752272

Arabidopsis thaliana

(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-3-hydroxy-9,19-cycloergosta-7,24(28)-diene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O

-

-

-

ir

(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-4-methylcholest-7-en-3-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+

-

752272

Arabidopsis thaliana

(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-3-hydroxycholest-7-ene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O

-

-

-

ir

24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+

-

752272

Arabidopsis thaliana

3beta,4alpha,5alpha-3-hydroxystigmasta-7,24(28)-diene-4-carboxylate + 6 ferricytochrome b5 + 4 H2O

-

-

-

?

24-methylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+

-

752272

Arabidopsis thaliana

3beta-hydroxyergosta-7,24(241)-dien-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O

-

-

-

ir

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

752271

Ramadan

Control of beta-sitosterol bi ...

Calotropis procera

Steroids

141

1-8

2019

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750668

Zhang

De novo sequencing and transc ...

Periploca sepium

Front. Plant Sci.

8

594

2017

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752272

Rahier

Dissecting the sterol C-4 dem ...

Arabidopsis thaliana

Steroids

76

340-352

2011

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1

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2

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657838

Darnet

Plant sterol biosynthesis: ide ...

Nicotiana benthamiana, Nicotiana tabacum, Arabidopsis thaliana

Biochem. J.

378

889-898

2004

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4

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16

4

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16

4

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750534

Darnet

Functional identification of ...

Arabidopsis thaliana

FEBS Lett.

508

39-43

2001

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749794

Rahier

The role of cytochrome b5, in ...

Zea mays

Biochem. Biophys. Res. Commun.

236

434-437

1997

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751005

Pascal

Plant sterol biosynthesis. Id ...

Zea mays

J. Biol. Chem.

268

11639-11654

1993

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6

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