Plant sterol biosynthesis. Identification and characterization of two distinct microsomal oxidative enzymatic systems involved in sterol C4-demethylation
Pascal, S.; Taton, M.; Rahier, A.; J. Biol. Chem. 268, 11639-11654 (1993) 
Data extracted from this reference:
Inhibitors
Inhibitors
Commentary
Organism
Structure
cyanide
-
Zea mays
cytochrome c
-
Zea mays
menadione
34% residual activity at 0.05 mM
Zea mays
additional information
not inhibited by N3-, CO (saturated), LAB 170250F, and tetcyclacis
Zea mays
orthophenanthroline
47% residual activity at 1 mM
Zea mays
stigmastan-3beta,5alpha,6alpha-triol
effectively inhibited by stigmastan-3beta,5alpha,6alpha-triol
Zea mays
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Zea mays
-
-
Organism
Organism
UniProt
Commentary
Textmining
Zea mays
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
-
751005
Zea mays
(3beta,4alpha,5alpha,24Z)-3-hydroxystigmasta-7,24(28)-diene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O
-
-
-
ir
24-ethylidenelophenol + NAD(P)H + O2 + H+
100% activity
751005
Zea mays
?
-
-
-
?
24-methylidenelophenol + NAD(P)H + H+
59% activity compared to 24-ethylidenelophenol
751005
Zea mays
?
-
-
-
?
24zeta(241)-dihydrocycloeucalenol + NAD(P)H + H+
10% activity compared to 24-ethylidenelophenol
751005
Zea mays
?
-
-
-
?
cycloeucalenol + NAD(P)H + H+
10% activity compared to 24-ethylidenelophenol
751005
Zea mays
?
-
-
-
?
lophenol + NAD(P)H + H+
38% activity compared to 24-ethylidenelophenol
751005
Zea mays
?
-
-
-
?
additional information
no activity with cyclobranol, cycloartenol, 24-methylenelanosterol, lanosterol, 24(25)-dihydrolanosterol, parkeol, 4,4-dimethyl-7,22-ergostadienol, beta-amyrine, 4alpha,14alpha,dimethyl-5alpha-cholesta-7-en-3beta-ol, obtusifoliol, 24zeta(241)-dihydroobtusifoliol, 29-norlanosterol, 24(25)-dihydro-29-norlanosterol, 4alpha-dimethyl-5alpha-cholestanol, 4alpha,14alpha,24zeta-trimethyl-5alpha-cholesta-9(11)-en-3beta-ol, and 4beta-24zeta(241)-dihydrocycloeucalenol
751005
Zea mays
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
4alpha-methyl oxidase
-
Zea mays
4alpha-methylsterol C4 oxidase
-
Zea mays
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
required
Zea mays
NADPH
required
Zea mays
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.035
-
using 24-ethylidenelophenol as substrate, at 30°C, pH not specified in the publication
Zea mays
cytochrome c
0.05
-
using 24-ethylidenelophenol as substrate, at 30°C, pH not specified in the publication
Zea mays
cyanide
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
required
Zea mays
NADPH
required
Zea mays
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.035
-
using 24-ethylidenelophenol as substrate, at 30°C, pH not specified in the publication
Zea mays
cytochrome c
0.05
-
using 24-ethylidenelophenol as substrate, at 30°C, pH not specified in the publication
Zea mays
cyanide
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cyanide
-
Zea mays
cytochrome c
-
Zea mays
menadione
34% residual activity at 0.05 mM
Zea mays
additional information
not inhibited by N3-, CO (saturated), LAB 170250F, and tetcyclacis
Zea mays
orthophenanthroline
47% residual activity at 1 mM
Zea mays
stigmastan-3beta,5alpha,6alpha-triol
effectively inhibited by stigmastan-3beta,5alpha,6alpha-triol
Zea mays
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Zea mays
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
-
751005
Zea mays
(3beta,4alpha,5alpha,24Z)-3-hydroxystigmasta-7,24(28)-diene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O
-
-
-
ir
24-ethylidenelophenol + NAD(P)H + O2 + H+
100% activity
751005
Zea mays
?
-
-
-
?
24-methylidenelophenol + NAD(P)H + H+
59% activity compared to 24-ethylidenelophenol
751005
Zea mays
?
-
-
-
?
24zeta(241)-dihydrocycloeucalenol + NAD(P)H + H+
10% activity compared to 24-ethylidenelophenol
751005
Zea mays
?
-
-
-
?
cycloeucalenol + NAD(P)H + H+
10% activity compared to 24-ethylidenelophenol
751005
Zea mays
?
-
-
-
?
lophenol + NAD(P)H + H+
38% activity compared to 24-ethylidenelophenol
751005
Zea mays
?
-
-
-
?
additional information
no activity with cyclobranol, cycloartenol, 24-methylenelanosterol, lanosterol, 24(25)-dihydrolanosterol, parkeol, 4,4-dimethyl-7,22-ergostadienol, beta-amyrine, 4alpha,14alpha,dimethyl-5alpha-cholesta-7-en-3beta-ol, obtusifoliol, 24zeta(241)-dihydroobtusifoliol, 29-norlanosterol, 24(25)-dihydro-29-norlanosterol, 4alpha-dimethyl-5alpha-cholestanol, 4alpha,14alpha,24zeta-trimethyl-5alpha-cholesta-9(11)-en-3beta-ol, and 4beta-24zeta(241)-dihydrocycloeucalenol
751005
Zea mays
?
-
-
-
?
Other publictions for EC 1.14.18.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752271
Ramadan
Control of beta-sitosterol bi ...
Calotropis procera
Steroids
141
1-8
2019
-
-
-
-
-
-
-
-
-
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2
-
1
-
-
-
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2
-
2
-
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1
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1
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2
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2
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750668
Zhang
De novo sequencing and transc ...
Periploca sepium
Front. Plant Sci.
8
594
2017
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4
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3
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2
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3
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752272
Rahier
Dissecting the sterol C-4 dem ...
Arabidopsis thaliana
Steroids
76
340-352
2011
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1
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1
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1
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4
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3
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1
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1
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4
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2
2
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657838
Darnet
Plant sterol biosynthesis: ide ...
Nicotiana benthamiana, Nicotiana tabacum, Arabidopsis thaliana
Biochem. J.
378
889-898
2004
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4
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1
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4
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4
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4
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16
4
8
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4
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4
4
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1
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4
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4
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16
4
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750534
Darnet
Functional identification of ...
Arabidopsis thaliana
FEBS Lett.
508
39-43
2001
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1
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1
1
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749794
Rahier
The role of cytochrome b5, in ...
Zea mays
Biochem. Biophys. Res. Commun.
236
434-437
1997
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2
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1
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6
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1
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2
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751005
Pascal
Plant sterol biosynthesis. Id ...
Zea mays
J. Biol. Chem.
268
11639-11654
1993
-
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6
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1
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1
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7
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2
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2
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2
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-
2
-
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-
2
6
-
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1
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7
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