Plant sterol biosynthesis: identification of two distinct families of sterol 4alpha-methyl oxidases
Darnet, S.; Rahier, A.; Biochem. J. 378, 889-898 (2004) 
Data extracted from this reference:
Cloned(Commentary)
Cloned (Commentary)
Organism
DNA and amino acid sequence determination and analysis
Nicotiana tabacum
DNA and amino acid sequence determination and analysis, expression in enzyme-lacking Saccharomyces cerevisiae strain erg25
Arabidopsis thaliana
DNA and amino acid sequence determination and analysis, in vitro transcription
Nicotiana benthamiana
Engineering
Protein Variants
Commentary
Organism
additional information
virus-iduced gene silencing approach for reduction of enzyme expression, sterol analysis of transgenic plants
Nicotiana benthamiana
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
non-heme iron
Arabidopsis thaliana
Iron
non-heme iron
Nicotiana benthamiana
Iron
non-heme iron
Nicotiana tabacum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Arabidopsis thaliana
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO
?
-
-
?
additional information
Nicotiana tabacum
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO
?
-
-
?
additional information
Nicotiana benthamiana
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO, sterol spectrum in wild-type and transgenic gene-silenced plants, accumulation of 4alpha-methyl-DELTA7-sterols, overview
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
Q8VWZ8
isozyme SMO2-2; 2 distinct enzyme families exist with 3 and 2 isozymes belonging to them, respectively
-
Arabidopsis thaliana
Q9ZW22
isozyme SMO2-1; 2 distinct enzyme families exist with 3 and 2 isozymes belonging to them, respectively
-
Nicotiana benthamiana
Q5J954
isozyme SMO2; 2 isozymes SMO1 and SMO2
-
Nicotiana tabacum
Q6YAU6
isozyme SMO2; cv BY2, isozymes SMO1 and SMO2
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
step 3 of 3 of the overall reaction
657838
Arabidopsis thaliana
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
-
-
-
?
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
step 3 of 3 of the overall reaction
657838
Nicotiana benthamiana
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
-
-
-
?
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
step 3 of 3 of the overall reaction
657838
Nicotiana tabacum
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
-
-
-
?
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
step 1 of 3 of the overall reaction
657838
Arabidopsis thaliana
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
-
-
-
?
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
step 1 of 3 of the overall reaction
657838
Nicotiana benthamiana
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
-
-
-
?
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
step 1 of 3 of the overall reaction
657838
Nicotiana tabacum
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
-
-
-
?
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
step 2 of 3 of the overall reaction
657838
Arabidopsis thaliana
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
-
-
-
?
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
step 2 of 3 of the overall reaction
657838
Nicotiana benthamiana
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
-
-
-
?
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
step 2 of 3 of the overall reaction
657838
Nicotiana tabacum
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
-
-
-
?
additional information
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO
657838
Arabidopsis thaliana
?
-
-
-
?
additional information
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO
657838
Nicotiana tabacum
?
-
-
-
?
additional information
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO, sterol spectrum in wild-type and transgenic gene-silenced plants, accumulation of 4alpha-methyl-DELTA7-sterols, overview
657838
Nicotiana benthamiana
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases
Arabidopsis thaliana
More
enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases
Nicotiana benthamiana
More
enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases
Nicotiana tabacum
Synonyms
Synonyms
Commentary
Organism
4alpha-methyl oxidase
-
Arabidopsis thaliana
4alpha-methyl oxidase
-
Nicotiana benthamiana
4alpha-methyl oxidase
-
Nicotiana tabacum
SMO2
-
Nicotiana benthamiana
SMO2
-
Nicotiana tabacum
SMO2-1
-
Arabidopsis thaliana
SMO2-2
-
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Arabidopsis thaliana
NAD(P)H
-
Nicotiana benthamiana
NAD(P)H
-
Nicotiana tabacum
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis
Nicotiana tabacum
DNA and amino acid sequence determination and analysis, expression in enzyme-lacking Saccharomyces cerevisiae strain erg25
Arabidopsis thaliana
DNA and amino acid sequence determination and analysis, in vitro transcription
Nicotiana benthamiana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Arabidopsis thaliana
NAD(P)H
-
Nicotiana benthamiana
NAD(P)H
-
Nicotiana tabacum
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
virus-iduced gene silencing approach for reduction of enzyme expression, sterol analysis of transgenic plants
Nicotiana benthamiana
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
non-heme iron
Arabidopsis thaliana
Iron
non-heme iron
Nicotiana benthamiana
Iron
non-heme iron
Nicotiana tabacum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Arabidopsis thaliana
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO
?
-
-
?
additional information
Nicotiana tabacum
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO
?
-
-
?
additional information
Nicotiana benthamiana
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO, sterol spectrum in wild-type and transgenic gene-silenced plants, accumulation of 4alpha-methyl-DELTA7-sterols, overview
?
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
step 3 of 3 of the overall reaction
657838
Arabidopsis thaliana
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
-
-
-
?
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
step 3 of 3 of the overall reaction
657838
Nicotiana benthamiana
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
-
-
-
?
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
step 3 of 3 of the overall reaction
657838
Nicotiana tabacum
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
-
-
-
?
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
step 1 of 3 of the overall reaction
657838
Arabidopsis thaliana
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
-
-
-
?
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
step 1 of 3 of the overall reaction
657838
Nicotiana benthamiana
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
-
-
-
?
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
step 1 of 3 of the overall reaction
657838
Nicotiana tabacum
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
-
-
-
?
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
step 2 of 3 of the overall reaction
657838
Arabidopsis thaliana
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
-
-
-
?
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
step 2 of 3 of the overall reaction
657838
Nicotiana benthamiana
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
-
-
-
?
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
step 2 of 3 of the overall reaction
657838
Nicotiana tabacum
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
-
-
-
?
additional information
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO
657838
Arabidopsis thaliana
?
-
-
-
?
additional information
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO
657838
Nicotiana tabacum
?
-
-
-
?
additional information
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO, sterol spectrum in wild-type and transgenic gene-silenced plants, accumulation of 4alpha-methyl-DELTA7-sterols, overview
657838
Nicotiana benthamiana
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases
Arabidopsis thaliana
More
enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases
Nicotiana benthamiana
More
enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases
Nicotiana tabacum
Other publictions for EC 1.14.18.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752271
Ramadan
Control of beta-sitosterol bi ...
Calotropis procera
Steroids
141
1-8
2019
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2
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1
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2
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750668
Zhang
De novo sequencing and transc ...
Periploca sepium
Front. Plant Sci.
8
594
2017
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752272
Rahier
Dissecting the sterol C-4 dem ...
Arabidopsis thaliana
Steroids
76
340-352
2011
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1
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1
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4
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2
2
-
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657838
Darnet
Plant sterol biosynthesis: ide ...
Nicotiana benthamiana, Nicotiana tabacum, Arabidopsis thaliana
Biochem. J.
378
889-898
2004
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4
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1
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4
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4
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4
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16
4
8
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4
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4
4
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1
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4
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4
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16
4
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750534
Darnet
Functional identification of ...
Arabidopsis thaliana
FEBS Lett.
508
39-43
2001
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1
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1
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749794
Rahier
The role of cytochrome b5, in ...
Zea mays
Biochem. Biophys. Res. Commun.
236
434-437
1997
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2
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751005
Pascal
Plant sterol biosynthesis. Id ...
Zea mays
J. Biol. Chem.
268
11639-11654
1993
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6
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2
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6
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7
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