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Literature summary for 1.14.18.10 extracted from
- Plant sterol biosynthesis. Identification and characterization of two distinct microsomal oxidative enzymatic systems involved in sterol C4-demethylation (1993), J. Biol. Chem., 268, 11639-11654 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | - |
Zea mays |  |
| cytochrome c | - |
Zea mays | |
| menadione | 39% residual activity at 0.05 mM | Zea mays | |
| additional information | not inhibited by N3-, CO (saturated), LAB 170250F, and tetcyclacis | Zea mays | |
| orthophenanthroline | 44% residual activity at 1 mM | Zea mays | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Zea mays | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 24-methylidenecycloartanol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ | Zea mays | overall reaction | 3beta-hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(241)-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 24(25)-dihydrocycloartenol + NAD(P)H + H+ | 30% activity compared to 24-methylidenecycloartanol | Zea mays | ? | - |
? | |
| 24-methylidenecycloartanol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ | overall reaction | Zea mays | 3beta-hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(241)-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O | - |
? | |
| 24-methylidenecycloartanol + NAD(P)H + O2 + H+ | 100% activity | Zea mays | 4alpha-hydroxymethyl-4beta,14alpha-dimethyl-5alpha-ergosta-9beta,19-cyclo-24(241)-en-3beta-ol + NAD(P)+ + H2O | - |
? | |
| 24zeta-methylcycloartanol + NAD(P)H + H+ | 43% activity compared to 24-methylidenecycloartanol | Zea mays | ? | - |
? | |
| cyclolaudenol + NAD(P)H + H+ | 56% activity compared to 24-methylidenecycloartanol | Zea mays | ? | - |
? | |
| additional information | no activity with cyclobranol, cycloartenol, 24-methylenelanosterol, lanosterol, 24(25)-dihydrolanosterol, parkeol, 4,4-dimethyl-7,22-ergostadienol, beta-amyrine, 4alpha,14alpha,dimethyl-5alpha-cholesta-7-en-3beta-ol, obtusifoliol, 24zeta(241)-dihydroobtusifoliol, 29-norlanosterol, 24(25)-dihydro-29-norlanosterol, 4alpha-dimethyl-5alpha-cholestanol, 4alpha,14alpha,24zeta-trimethyl-5alpha-cholesta-9(11)-en-3beta-ol, and 4beta-24zeta(241)-dihydrocycloeucalenol | Zea mays | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4,4-dimethylsterol-oxidase | - |
Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | required | Zea mays | |
| NADPH | required | Zea mays | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
using 24-methylenecycloartanol as substrate, at 30°C, pH not specified in the publication | Zea mays | cyanide | |
| 0.03 | - |
using 24-methylenecycloartanol as substrate, at 30°C, pH not specified in the publication | Zea mays | cytochrome c | |
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