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Literature summary for 1.14.18.1 extracted from

  • Feng, X.; Liu, F.; Lin, C.; Lin, X.; Liu, N.; Wang, X.
    Purification and characterization of polyphenol oxidase from the bud of Lonicera confusa (2014), J. Food Biochem., 38, 533-541 .
No PubMed abstract available
Search for more literature for 1.14.18.1

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid strong inhibitor, 78% inhibition at 1 mM, 88% inhibition at 10 mM Lonicera confusa
Citric acid 12% inhibition at 1 mM, 60% inhibition at 10 mM Lonicera confusa
Cu2+ 52% inhibition at 1 mM, 58% inhibition at 10 mM Lonicera confusa
EDTA no inhibition at 1 mM, 17% inhibition at 10 mM Lonicera confusa
glutathione 52% inhibition at 1 mM, 56% inhibition at 10 mM Lonicera confusa
kojic acid 55% inhibition at 1 mM, 86% inhibition at 10 mM Lonicera confusa
additional information poor inhibition by sodium chloride at 10 mM Lonicera confusa
Sodium metabisulfite 67% inhibition at 1 mM, 70% inhibition at 10 mM Lonicera confusa
sodium sulfite 66% inhibition at 1 mM, 70% inhibition at 10 mM Lonicera confusa
tropolone 48% inhibition at 1 mM, 64% inhibition at 10 mM Lonicera confusa
Zn2+ activates by 6% at 5 mM and inhibits by 34% at 10 mM Lonicera confusa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaeli-Menten kinetics Lonicera confusa
0.037
-
 caffeic acid pH 8.5, 15°C Lonicera confusa
0.071
-
 gallate pH 8.5, 15°C Lonicera confusa
0.11
-
 pyrogallate pH 8.5, 15°C Lonicera confusa
0.13
-
 methylgallate pH 8.5, 15°C Lonicera confusa
0.28
-
 catechol pH 8.5, 15°C Lonicera confusa
2.26
-
 L-Dopa pH 8.5, 15°C Lonicera confusa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates by 34% at 5 mM and 45% at 10 mM Lonicera confusa
Cu2+ a copper-containing enzyme, exogenous Cu2+ inhibits the enzyme by avbout 55% at 1-10 mM Lonicera confusa
Mg2+ activates by 46% at 5 mM and 59% at 10 mM Lonicera confusa
Mn2+ activates by 76% at 5 mM and 101% at 10 mM Lonicera confusa
Zn2+ activates by 6% at 5 mM and inhibits by 34% at 10 mM Lonicera confusa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28800
-
 gel filtration combined with multiangle laser light scattering Lonicera confusa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 catechol + O2 Lonicera confusa
-
 2 1,2-benzoquinone + 2 H2O
-
 ?
2 L-dopa + O2 Lonicera confusa
-
 2 dopaquinone + 2 H2O
-
 ?
2 pyrocatechol + O2 Lonicera confusa
-
 ?
-
 ?
L-tyrosine + O2 Lonicera confusa
-
 dopaquinone + H2O
-
 ?
additional information Lonicera confusa the PPO from Lonicera confusa exhibits both diphenolase and triphenolase activities, substrates monophenol (L-tyrosine), diphenols (L-DOPA, catechol, caffeic acid) and triphenols (pyrogallic acid, methyl gallate and gallic acid) are used by the enzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lonicera confusa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from flower buds 20.27fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography Lonicera confusa

Source Tissue

Source Tissue Comment Organism Textmining
flower bud
-
 Lonicera confusa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
111.75
-
 crude enzyme extract, pH 8.5, 15°C Lonicera confusa
2847.22
-
 purified enzyme, pH 8.5, 15°C Lonicera confusa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 catechol + O2
-
 Lonicera confusa 2 1,2-benzoquinone + 2 H2O
-
 ?
2 L-dopa + O2
-
 Lonicera confusa 2 dopaquinone + 2 H2O
-
 ?
2 pyrocatechol + O2
-
 Lonicera confusa ?
-
 ?
caffeic acid + O2
-
 Lonicera confusa ? + 2 H2O
-
 ?
gallate + O2
-
 Lonicera confusa ? + 2 H2O
-
 ?
L-tyrosine + O2
-
 Lonicera confusa dopaquinone + H2O
-
 ?
methylgallate + O2
-
 Lonicera confusa ? + 2 H2O
-
 ?
additional information the PPO from Lonicera confusa exhibits both diphenolase and triphenolase activities, substrates monophenol (L-tyrosine), diphenols (L-DOPA, catechol, caffeic acid) and triphenols (pyrogallic acid, methyl gallate and gallic acid) are used by the enzyme Lonicera confusa ?
-
 ?
pyrogallate + O2
-
 Lonicera confusa ? + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 28800, SDS-PAGE Lonicera confusa

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
 Lonicera confusa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
-
 Lonicera confusa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 20 purified native enzyme, 60 min, over 90% activity remaining Lonicera confusa
40 60 purified native enzyme, 60 min, loss of 50% activity Lonicera confusa
60
-
 purified native enzyme, 10 min, loss of 30% activity Lonicera confusa
70 80 purified native enzyme, 10 min, loss of over 70% activity, inactivation Lonicera confusa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Lonicera confusa

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 at pH below 6.0, PPO is inactivated. The PPO activity rapidly increases with pH in the range of 6.5-8.5, above pH 8.5, the activity decreases to about 80% of maximal activity at pH 9.0-10.0 Lonicera confusa

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 more than 80% of maximal activity is maintained between pH 9.0 and pH 10.0, high activity in the pH range of 8.0-10.0 Lonicera confusa