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Literature summary for 1.14.18.1 extracted from

  • Patil, S.; Sistla, S.; Jadhav, J.
    Screening of inhibitors for mushroom tyrosinase using surface plasmon resonance (2014), J. Agric. Food Chem., 62, 11594-11601 .
    View publication on PubMed
Search for more literature for 1.14.18.1

Application

Application Comment Organism
drug development the regulation of melanin synthesis via the inhibition of tyrosinase is a research topic in the context of preventing hyperpigmentation, thus tyrosinase inhibitors are increasingly important in the food, medical, and cosmetic industries. The evaluated suface plasmon resonance biosensor has the potential to be used in the detection and screening of inhibitor drug candidates Agaricus bisporus

Inhibitors

Inhibitors Comment Organism Structure
catechol enzyme-inhibitor interaction measurement by SPR Agaricus bisporus
additional information inhibitor screening using surface plasmon resonance (SPR), two-state modeling. Structural changes of the mushroom tyrosinase in the presence of inhibitors are analyzed by circular dichroism spectroscopy Agaricus bisporus
phloroglucinol enzyme-inhibitor interaction measurement by SPR Agaricus bisporus
pyrogallol enzyme-inhibitor interaction measurement by SPR Agaricus bisporus
saffron enzyme-inhibitor interaction measurement by SPR Agaricus bisporus
Tannic acid enzyme-inhibitor interaction measurement by SPR Agaricus bisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-dopa + O2 Agaricus bisporus
-
 2 dopaquinone + 2 H2O
-
 ?
L-tyrosine + O2 Agaricus bisporus
-
 dopaquinone + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Agaricus bisporus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Agaricus bisporus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-dopa + O2
-
 Agaricus bisporus 2 dopaquinone + 2 H2O
-
 ?
L-tyrosine + O2
-
 Agaricus bisporus dopaquinone + H2O
-
 ?
additional information monophenolase and diphenolase activities of mushroom tyrosinase are performed using L-tyrosine and L-DOPA, respectively, by measuring the dopachrome accumulation at 475 nm before immobilization on the chip surface for surface plasmon resonance analysis Agaricus bisporus ?
-
 ?

Synonyms

Synonyms Comment Organism
mushroom tyrosinase
-
 Agaricus bisporus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Agaricus bisporus

General Information

General Information Comment Organism
physiological function tyrosinase is a multifunctional copper-containing oxygenase and catalyzes the hydroxylation of a monophenol and the conversion of o-diphenols to the corresponding o-quinones. These quinones are highly reactive compounds that can polymerize spontaneously to form melanin. Products formed as a result of tyrosinase activity are precursors for skin pigmentation, defense, and protective mechanisms in fungi Agaricus bisporus