Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | - |
Macrolepiota gracilenta | |
benzoic acid | - |
Macrolepiota gracilenta | |
Hg2+ | Hg2+ causes an inhibition in the range of 13% and 72% at 1 and 10 mM, respectively | Macrolepiota gracilenta | |
L-cysteine | - |
Macrolepiota gracilenta | |
L-phenylalanine | - |
Macrolepiota gracilenta | |
Ni2+ | 87% inhibition at 10 mM | Macrolepiota gracilenta | |
Sodium azide | - |
Macrolepiota gracilenta | |
Sodium metabisulfite | - |
Macrolepiota gracilenta | |
Thiourea | - |
Macrolepiota gracilenta | |
Zn2+ | 82% inhibition at 10 mM | Macrolepiota gracilenta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
3,4-dihydroxyhydrocinnamic acid | at pH 7.0 and 22°C | Macrolepiota gracilenta | |
1 | - |
4-hydroxyphenylpropionic acid | at pH 7.0 and 22°C | Macrolepiota gracilenta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Macrolepiota gracilenta | |
Mn2+ | - |
Macrolepiota gracilenta | |
Ni2+ | - |
Macrolepiota gracilenta | |
Zn2+ | - |
Macrolepiota gracilenta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57300 | - |
x * 57300, estimated from SDS-PAGE | Macrolepiota gracilenta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-DOPA + O2 | Macrolepiota gracilenta | 1% activity compared to 3,4-dihydroxyhydrocinnamic acid | dopaquinone + H2O | - |
? | |
L-tyrosine + O2 | Macrolepiota gracilenta | 6% activity compared to 3,4-dihydroxyhydrocinnamic acid | dopaquinone + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macrolepiota gracilenta | - |
- |
- |
Purification (Comment) | Organism |
---|---|
acetone precipitation, Sepharose 4B-L-tyrosine-p-amino benzoic acid affinity column chromatography | Macrolepiota gracilenta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0402 | - |
crude extract, at pH 7.0 and 22°C | Macrolepiota gracilenta |
2.625 | - |
after 65.3fold purification, at pH 7.0 and 22°C | Macrolepiota gracilenta |
Storage Stability | Organism |
---|---|
4°C, pH 5.0/7.0, 5 days, monophenolase/diphenolase activities of the enzyme are conserved approximately 40% and 60%, respectively | Macrolepiota gracilenta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyhydrocinnamic acid + O2 | 100% activity | Macrolepiota gracilenta | ? | - |
? | |
4-hydroxyphenylpropionic acid + O2 | 85% activity compared to 3,4-dihydroxyhydrocinnamic acid | Macrolepiota gracilenta | ? | - |
? | |
4-methylcatechol + O2 | 90% activity compared to 3,4-dihydroxyhydrocinnamic acid | Macrolepiota gracilenta | ? | - |
? | |
catechol + O2 | 12% activity compared to 3,4-dihydroxyhydrocinnamic acid | Macrolepiota gracilenta | ? | - |
? | |
L-DOPA + O2 | 1% activity compared to 3,4-dihydroxyhydrocinnamic acid | Macrolepiota gracilenta | dopaquinone + H2O | - |
? | |
L-tyrosine + O2 | 6% activity compared to 3,4-dihydroxyhydrocinnamic acid | Macrolepiota gracilenta | dopaquinone + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57300, estimated from SDS-PAGE | Macrolepiota gracilenta |
Synonyms | Comment | Organism |
---|---|---|
cresolase | - |
Macrolepiota gracilenta |
monophenol monooxygenase | - |
Macrolepiota gracilenta |
polyphenol oxidase | - |
Macrolepiota gracilenta |
PPO | - |
Macrolepiota gracilenta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
temperature optimum for diphenolase activity | Macrolepiota gracilenta |
40 | - |
temperature optimum for monophenolase activity | Macrolepiota gracilenta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the diphenolase activity is more sensitive to temperatures above 40°C. Monophenolase activity conserves approximately 85% at 50°C. Diphenolase activity maintains its original activity 70% at 30°C at pH 5.0 after 1 day of incubation. The enzyme, for both activities, is completely inactivated at 60°C after 4 h | Macrolepiota gracilenta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
pH optimum for diphenolase activity | Macrolepiota gracilenta |
7 | - |
pH optimum for monophenolase activity | Macrolepiota gracilenta |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
pH 7.0, the enzyme retains the diphenolase activity 80% at 4°C and 40% at 30°C. The enzyme loses diphenolase activity at all pH value sat 30°C after 5 day incubation | Macrolepiota gracilenta |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.008 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | Thiourea | |
0.03 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | ascorbic acid | |
0.06 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | Sodium metabisulfite | |
0.085 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | Thiourea | |
0.178 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | Sodium metabisulfite | |
0.24 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | L-cysteine | |
0.26 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | ascorbic acid | |
0.3 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | L-cysteine | |
0.5 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | Sodium azide | |
0.9 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | benzoic acid | |
3.56 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | L-phenylalanine | |
4.33 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | benzoic acid | |
13.8 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | Sodium azide | |
16 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | L-phenylalanine |