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Literature summary for 1.14.18.1 extracted from
- Purification and biochemical characterization of ionically unbound polyphenol oxidase from Musa paradisiaca leaf (2011), Prep. Biochem. Biotechnol., 41, 187-200.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ferrulic acid | enhances enzyme activity | Musa x paradisiaca |  |
| protocatechuate | enhances enzyme activity | Musa x paradisiaca | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Musa x paradisiaca | |
| 5-hydroxy-2-(hydroxymethyl)-4H-pyran-4-one | - |
Musa x paradisiaca | |
| ascorbic acid | - |
Musa x paradisiaca | |
| Co2+ | - |
Musa x paradisiaca | |
| EDTA | - |
Musa x paradisiaca | |
| K+ | - |
Musa x paradisiaca | |
| L-cysteine | - |
Musa x paradisiaca | |
| Na+ | - |
Musa x paradisiaca | |
| Sodium azide | - |
Musa x paradisiaca | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
catechol | pH 7.0, 50°C | Musa x paradisiaca | |
| 10 | - |
4-methylcatechol | pH 7.0, 50°C | Musa x paradisiaca | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | enhances enzyme activity | Musa x paradisiaca | |
| Fe2+ | enhances enzyme activity | Musa x paradisiaca | |
| Hg2+ | enhances enzyme activity | Musa x paradisiaca | |
| Mn2+ | enhances enzyme activity | Musa x paradisiaca | |
| Ni2+ | enhances enzyme activity | Musa x paradisiaca | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Musa x paradisiaca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Musa x paradisiaca | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and Sephadex G-100 gel filtration | Musa x paradisiaca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Musa x paradisiaca | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 catechol + O2 | - |
Musa x paradisiaca | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 4-methylcatechol + O2 | - |
Musa x paradisiaca | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40000, SDS-PAGE | Musa x paradisiaca |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| polyphenol oxidase | - |
Musa x paradisiaca |
| PPO | - |
Musa x paradisiaca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Musa x paradisiaca |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 90 | - |
Musa x paradisiaca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 75 | in the range from 60 to 75°C, the half-life of the enzyme reaches from 17 to 77 min | Musa x paradisiaca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Musa x paradisiaca |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | - |
Musa x paradisiaca |
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