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Literature summary for 1.14.18.1 extracted from

  • Cai, G.; Bae, Y.; Zhang, Y.; He, Y.; Jiang, M.; He, L.
    Expression and characterization of two tyrosinases from the trematode Schistosoma japonicum (2009), Parasitol. Res., 104, 601-609.
    View publication on PubMed
Search for more literature for 1.14.18.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Schistosoma japonicum

Inhibitors

Inhibitors Comment Organism Structure
allyl thiourea
-
 Schistosoma japonicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 mature form containing the two copper binding domains, SDS-PAGE Schistosoma japonicum
55000
-
 isozyme TYR1, calculated from amino acid sequence Schistosoma japonicum
56800
-
 isozyme TYR2, calculated from amino acid sequence Schistosoma japonicum

Organism

Organism UniProt Comment Textmining
Schistosoma japonicum Q5DB10
-
-
Schistosoma japonicum Q5DHS0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Schistosoma japonicum

Source Tissue

Source Tissue Comment Organism Textmining
adult isozyme TYR1 is strictly transcribed in the mature adult female worm, the transcript is not expressed in cercariae stage, the gene of the adult male worm shows very low transcription levels Schistosoma japonicum
-
adult isozyme TYR2 is strictly transcribed in the mature adult female worm, the transcript is not expressed in cercariae stage, the gene of the adult male worm shows very low transcription levels Schistosoma japonicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 catechol + O2
-
 Schistosoma japonicum 2 1,2-benzoquinone + 2 H2O
-
 ?
L-tyrosine methyl ester + O2
-
 Schistosoma japonicum L-DOPA methyl ester + H2O
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Schistosoma japonicum

Synonyms

Synonyms Comment Organism
TYR1 isozyme Schistosoma japonicum
TYR2 isozyme Schistosoma japonicum
tyrosinase
-
 Schistosoma japonicum

pI Value

Organism Comment pI Value Maximum pI Value
Schistosoma japonicum isozyme TYR1, calculated from amino acid sequence
-
 8.9
Schistosoma japonicum isozyme TYR2, calculated from amino acid sequence
-
 9

General Information

General Information Comment Organism
physiological function tyrosinases might play a significant role during egg shell formation in Schistosoma japonicum Schistosoma japonicum