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Literature summary for 1.14.18.1 extracted from
- Differential in vitro inhibition of polyphenoloxidase from a wild edible mushroom Lactarius salmonicolor (2009), J. Enzyme Inhib. Med. Chem., 24, 464-470.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sulfosalicylic acid | - |
Lactarius salmonicolor |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-mercaptoethanol | uncompetitive inhibition | Lactarius salmonicolor | |
| glutathione | mixed-type inhibition | Lactarius salmonicolor | |
| L-cysteine | uncompetitive inhibition | Lactarius salmonicolor | |
| L-tyrosine | uncompetitive inhibition | Lactarius salmonicolor | |
| oxalic acid | uncompetitive inhibition | Lactarius salmonicolor | |
| p-aminobenzenesulfonamide | competitive inhibition | Lactarius salmonicolor | |
| syringic acid | uncompetitive inhibition | Lactarius salmonicolor | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactarius salmonicolor | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and Sepharose 4B-L-tyrosine-p-amino benzoic acid affinity column chromatography | Lactarius salmonicolor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the crude extract has a specific activity of 4654.2 units/mg and the 26.6fold purified enzyme has a specific activity of 123809.5 units/mg, one unit is defined as the amount of enzyme that causes an increase of absorbance of 0.001 unit/min for 1 ml of enzyme at 25°C | Lactarius salmonicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 catechol + O2 | - |
Lactarius salmonicolor | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 4-methylcatechol + O2 | - |
Lactarius salmonicolor | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
| L-tyrosine + O2 | - |
Lactarius salmonicolor | dopaquinone + H2O | - |
? | |
| pyrogallol + O2 | - |
Lactarius salmonicolor | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| polyphenoloxidase | - |
Lactarius salmonicolor |
| PPO | - |
Lactarius salmonicolor |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 50 | at the shortest treatment time (5 min) the highest activity is seen at 5°C | Lactarius salmonicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | activity is reasonably maintained after 40 min of incubation at temperature below 30°C, significant inactivation becomes apparent at 40°C, and more so at 50°C, enzyme activity decreases due to heat denaturation with increasing temperature | Lactarius salmonicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
using 4-methylcatechol as substrate | Lactarius salmonicolor |
| 7 | - |
using pyrogallol as substrate | Lactarius salmonicolor |
| 7.5 | - |
using catechol as substrate | Lactarius salmonicolor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 11 | - |
Lactarius salmonicolor |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000743 | - |
oxalic acid | at 25°C | Lactarius salmonicolor | |
| 0.0067 | - |
glutathione | competitive inhibition, at 25°C | Lactarius salmonicolor | |
| 0.0214 | - |
syringic acid | at 25°C | Lactarius salmonicolor | |
| 0.031 | - |
beta-mercaptoethanol | at 25°C | Lactarius salmonicolor | |
| 0.145 | - |
L-cysteine | at 25°C | Lactarius salmonicolor | |
| 0.16 | - |
glutathione | noncompetitive inhibition, at 25°C | Lactarius salmonicolor | |
| 0.267 | - |
p-aminobenzenesulfonamide | at 25°C | Lactarius salmonicolor | |
| 1.75 | - |
L-tyrosine | at 25°C | Lactarius salmonicolor | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00038 | - |
at 25°C | Lactarius salmonicolor | L-tyrosine | |
| 0.12 | - |
at 25°C | Lactarius salmonicolor | oxalic acid | |
| 0.15 | - |
at 25°C | Lactarius salmonicolor | L-cysteine | |
| 0.23 | - |
at 25°C | Lactarius salmonicolor | p-aminobenzenesulfonamide | |
| 0.4 | - |
at 25°C | Lactarius salmonicolor | syringic acid | |
| 0.49 | - |
at 25°C | Lactarius salmonicolor | beta-mercaptoethanol | |
| 0.91 | - |
at 25°C | Lactarius salmonicolor | glutathione | |
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