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Literature summary for 1.14.18.1 extracted from

  • Selinheimo, E.; Gasparetti, C.; Mattinen, M.; Steffensen, C.; Buchert, J.; Kruus, K.
    Comparison of substrate specificity of tyrosinases from Trichoderma reesei and Agaricus bisporus (2009), Enzyme Microb. Technol., 44, 1-10.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
p-tyrosol in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
0.11
-
Gly-Gly-L-Tyr in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
0.17
-
L-Dopa in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
0.18
-
Gly-L-Tyr-Gly in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
0.2
-
L-tyrosine in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
0.21
-
L-Tyr-Gly-Gly in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
0.25
-
catechol in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
0.33
-
phenol in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
0.64
-
phloretic acid in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
0.75
-
tyramine in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
0.8
-
p-tyrosol in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
0.84
-
dopamine in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
0.9
-
caffeic acid in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
0.91
-
Hydrocaffeic acid in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.4
-
phloretic acid in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.5
-
p-coumaric acid in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.69
-
caffeic acid in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
2.5
-
catechol in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
3
-
Hydrocaffeic acid in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
3.1
-
Gly-Gly-L-Tyr in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
3.8
-
phenol in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
3.9
-
Gly-L-Tyr-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
4.5
-
tyramine in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
6
-
L-Tyr-Gly-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
7.5
-
L-Dopa in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
11
-
dopamine in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Agaricus bisporus
-
-
-
Trichoderma reesei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 catechol + O2
-
Agaricus bisporus 2 1,2-benzoquinone + 2 H2O
-
?
2 catechol + O2
-
Trichoderma reesei 2 1,2-benzoquinone + 2 H2O
-
?
caffeic acid + O2
-
Agaricus bisporus caffeoyl quinone + H2O
-
?
caffeic acid + O2
-
Trichoderma reesei caffeoyl quinone + H2O
-
?
dopamine + O2
-
Agaricus bisporus ?
-
?
dopamine + O2
-
Trichoderma reesei ?
-
?
Gly-Gly-L-Tyr + O2
-
Agaricus bisporus ?
-
?
Gly-Gly-L-Tyr + O2
-
Trichoderma reesei ?
-
?
Gly-L-Tyr-Gly + O2
-
Agaricus bisporus ?
-
?
Gly-L-Tyr-Gly + O2
-
Trichoderma reesei ?
-
?
hydrocaffeic acid + O2
-
Agaricus bisporus ?
-
?
hydrocaffeic acid + O2
-
Trichoderma reesei ?
-
?
L-DOPA + O2
-
Agaricus bisporus dopaquinone + H2O
-
?
L-DOPA + O2
-
Trichoderma reesei dopaquinone + H2O
-
?
L-Tyr-Gly-Gly + O2
-
Agaricus bisporus ?
-
?
L-Tyr-Gly-Gly + O2
-
Trichoderma reesei ?
-
?
L-tyrosine + O2
-
Agaricus bisporus L-DOPA + H2O
-
?
L-tyrosine + O2
-
Trichoderma reesei L-DOPA + H2O
-
?
p-coumaric acid + O2
-
Agaricus bisporus caffeic acid + H2O
-
?
p-coumaric acid + O2
-
Trichoderma reesei caffeic acid + H2O
-
?
p-tyrosol + O2
-
Agaricus bisporus 2-(3,4-dihydroxyphenyl)ethanol + H2O
-
?
p-tyrosol + O2
-
Trichoderma reesei 2-(3,4-dihydroxyphenyl)ethanol + H2O
-
?
phenol + O2
-
Agaricus bisporus ?
-
?
phenol + O2
-
Trichoderma reesei ?
-
?
phloretic acid + O2
-
Agaricus bisporus ?
-
?
phloretic acid + O2
-
Trichoderma reesei ?
-
?
tyramine + O2
-
Agaricus bisporus ?
-
?
tyramine + O2
-
Trichoderma reesei ?
-
?

Synonyms

Synonyms Comment Organism
monophenol, o-diphenol:oxygen oxidoreductase
-
Agaricus bisporus
monophenol, o-diphenol:oxygen oxidoreductase
-
Trichoderma reesei
tyrosinase
-
Agaricus bisporus
tyrosinase
-
Trichoderma reesei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
Gly-Gly-L-Tyr in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
3.1
-
Gly-L-Tyr-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
3.2
-
L-Tyr-Gly-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
4
-
L-Tyr-Gly-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
4.2
-
Gly-L-Tyr-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
7.2
-
Gly-Gly-L-Tyr in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.5
-
L-Tyr-Gly-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.1
-
glycyl-L-tyrosylglycine in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
2.4
-
Gly-Gly-L-Tyr in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
17.1
-
Gly-L-Tyr-Gly in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
19
-
L-Tyr-Gly-Gly in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
26.5
-
Gly-Gly-L-Tyr in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus