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Literature summary for 1.14.18.1 extracted from
- Some kinetic properties of polyphenol oxidase obtained from dill (Anethum graveolens) (2008), J. Enzyme Inhib. Med. Chem., 23, 380-385.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | the enzyme is a target for development of specific inhibitors to avoid unfavorable enzymatic browning of plant-derived foods. The inhibitors used in this study can be used safely in making herb cheese | Anethum graveolens |
General Stability
| General Stability | Organism |
|---|---|
| The stability of the enzyme is determined by measuring activity in the various buffers (0.1 M citrate/0.2 M phosphate for pH 3.5-5.0, 0.2 M phosphate for pH 5.5-7.5) every 3 days using catechol as substrate under optimum conditions (pH, temperature and ionic strength). | Anethum graveolens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | noncompetitve | Anethum graveolens |  |
| glutathione | noncompetitve | Anethum graveolens | |
| L-cysteine | most effective inhibitor, noncompetitve | Anethum graveolens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.835 | - |
chlorogenic acid | PPO of dill has a great affinity towards chlorogenic acid. calculated from the Lineweaver-Burk graphs for substrates | Anethum graveolens | |
| 1.67 | - |
dopamine | calculated from the Lineweaver-Burk graphs for substrates | Anethum graveolens | |
| 2.17 | - |
catechol | calculated from the Lineweaver-Burk graphs for substrates | Anethum graveolens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anethum graveolens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially purified from dill by (NH4)2SO4 percipititation followed by dialysis and gel filtration chromatography | Anethum graveolens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | kept for 2 days in the refrigerator at 4°C before PPO extraction | Anethum graveolens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity is measured spectrophotometrically at 420 nm, 13530.07 EU/mg of protein, gel filtration chromatography | Anethum graveolens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| catechol + 1/2 O2 | - |
Anethum graveolens | 1,2-benzoquinone + H2O | - |
? | |
| chlorogenic acid + 1/2 O2 | best substrate | Anethum graveolens | chlorogenoquinone + H2O | - |
? | |
| dopamine + 1/2 O2 | - |
Anethum graveolens | dopamine quinone + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| polyphenol oxidase | responsible for browning reaction in demaged plant tissues and fruits | Anethum graveolens |
| PPO | - |
Anethum graveolens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | - |
substrate-dependent, with catechol as a subsrate | Anethum graveolens |
| 10 | - |
substrate-dependent, with chorogenic acid as a subsrate | Anethum graveolens |
| 55 | - |
substrate-dependent, with dopamine as a subsrate | Anethum graveolens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
85 | the reaction is carried out at various temperatures and pH values with the substrates catechol, dopamine and chorogenic acid. PPO activity is measured at different temperatures in the range from 0 to 85°C using the 3 different substrates to determine the optimum temperature of the enzyme | Anethum graveolens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
with chorogenic acid as a substrate, phosphate buffer | Anethum graveolens |
| 6.5 | - |
with catechol as a substrate, phosphate buffer. PPO activity is highest at pH 6.5, but decreases at similar rates at each of the pH values studied | Anethum graveolens |
| 8.5 | - |
with dopamine as a substrate, Tris-HCl buffer | Anethum graveolens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the reaction is carried out at various temperatures and pH values with the substrates catechol, dopamine and chorogenic acid | Anethum graveolens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
L-cysteine | - |
Anethum graveolens | |
| 0.0289 | - |
L-cysteine | - |
Anethum graveolens | |
| 0.0292 | - |
L-cysteine | - |
Anethum graveolens | |
| 0.0473 | - |
ascorbic acid | - |
Anethum graveolens | |
| 0.0647 | - |
ascorbic acid | - |
Anethum graveolens | |
| 0.0692 | - |
ascorbic acid | - |
Anethum graveolens | |
| 0.0809 | - |
glutathione | - |
Anethum graveolens | |
| 0.084 | - |
glutathione | - |
Anethum graveolens | |
| 0.0897 | - |
glutathione | - |
Anethum graveolens | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0204 | - |
- |
Anethum graveolens | L-cysteine | |
| 0.0325 | - |
- |
Anethum graveolens | glutathione | |
| 0.0426 | - |
- |
Anethum graveolens | ascorbic acid | |
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