Cloned (Comment) | Organism |
---|---|
tyrosinase gene transfection, by calcium-phosphate precipitation method, into HEK293 cell significantly down-regulates the expression levels of solute carrier family 12, member 4 and 7, which are known to be Cl- transporters. Observation of significant pigmentation of the HEK293 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cl- | enzyme added to substrate L-dopa, that is mixed with various concentrations of NaCl in 0.05 M sodium phosphate buffer, pH 7.0. When the NaCl concentration reaches to 600 mM, almost all the enzyme activity is abolished. Cl- induced inhibition of human tyrosinase, Cl- inhibits tyrosinase in a slope-parabolic competitive manner. TXM13 originated tyrosinase and the overexpressed human tyrosinase in HEK293 cells are inhibited. The inhibition by Cl- is reversible | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
L-Dopa | human tyrosinase source HEK293-TYR | Homo sapiens | |
0.49 | - |
L-Dopa | human tyrosinase source TXM13 pigment cell | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | The immunocytochemistry shows that the ectopic tyrosinase is located in the cytoplasm, it is propably the membrane-bounded form since no detection of enzyme activity with the soluble solution without Triton X-100 is possible, nor is it found in Western blotting | Homo sapiens | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
TXM13 cell | prepared from the TXM13 melanotic cells as well as from cells that had undergone gene transfection | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-dihydroxyindole + 1/2 O2 | - |
Homo sapiens | 1H-indole-5,6-dione + H2O | - |
? | |
L-dopa + 1/2 O2 | - |
Homo sapiens | L-dopaquinone + H2O | - |
? | |
L-tyrosine + O2 + AH2 | - |
Homo sapiens | L-dopa + H2O + A | - |
? |
Synonyms | Comment | Organism |
---|---|---|
monophenol, dihydroxyphenylalanine:oxygen oxidoreductase | copper binding metalloenzyme | Homo sapiens |
tyrosinase | plays a core role in melanogenesis | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
Cl- | human tyrosinase source TXM13 pigment cell | Homo sapiens | |
38.9 | - |
Cl- | human tyrosinase source HEK293-TYR | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
155 | - |
human tyrosinase source TXM13 pigment cell | Homo sapiens | Cl- | |
164 | - |
human tyrosinase source HEK293-TYR | Homo sapiens | Cl- |