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Literature summary for 1.14.18.1 extracted from

  • Otavio de Faria, R.; Moure, V.R.; Balmant, W.; Lopes de Almeida Amazonas, M.A.; Krieger, N.; Mitchell, D.A.
    The tyrosinase produced by Lentinula boryana (Berk. & Mont.) Pegler suffers substrate inhibition by L-DOPA (2007), Food Technol. Biotechnol., 45, 334-340.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
L-Dopa
-
Lentinula boryana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
L-Dopa preparation shows substrate inhibition kinetics with L-dopa (3,4-dihydroxy-L-phenylalanine) Lentinula boryana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
the purified preparation gives three bands, 1 * 20000 + 1 * 27000 + 1 * 47000, SDS-PAGE Lentinula boryana
27000
-
the purified preparation gives three bands, 1 * 20000 + 1 * 27000 + 1 * 47000, SDS-PAGE Lentinula boryana
47000
-
the purified preparation gives three bands, 1 * 20000 + 1 * 27000 + 1 * 47000, SDS-PAGE Lentinula boryana

Organism

Organism UniProt Comment Textmining
Lentinula boryana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified with ammonium sulfate precipitation followed by ion exchange chromatography on a DEAE Sepharose column, obtaining a yield of 33% and a 5.3fold enrichment Lentinula boryana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-dopa + 1/2 O2
-
Lentinula boryana L-dopaquinone + H2O
-
?

Subunits

Subunits Comment Organism
More the purified preparation gives three bands, 1 * 20000 + 1 * 27000 + 1 * 47000, SDS-PAGE Lentinula boryana

Synonyms

Synonyms Comment Organism
catecholase
-
Lentinula boryana
Diphenol oxidase
-
Lentinula boryana
L-DOPA:oxygen oxidoreductase
-
Lentinula boryana
polyphenol oxidase
-
Lentinula boryana
tyrosinase
-
Lentinula boryana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life 10 min Lentinula boryana
60
-
half-life 1 min Lentinula boryana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Lentinula boryana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
72
-
L-Dopa
-
Lentinula boryana