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Literature summary for 1.14.18.1 extracted from
- Phenolic substrates and suicide inactivation of tyrosinase: kinetics and mechanism (2008), Biochem. J., 416, 431-440.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | study of suicide inactivation and irreversible inhibition is important in the functional design of synthetic inactivators for therapeutic applications | Agaricus bisporus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | suicide inactivation of tyrosinase acting on o-diphenols. A kinetic study of the suicide inactivation of tyrosinase during its action on a variety of substrates is described, and a mechanism is proposed to explain the experimental kinetic results and to throw light on the suicide inactivation step of mushroom tyrosinase | Agaricus bisporus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
protocatechuic acid | - |
Agaricus bisporus |  |
| 0.1 | - |
4-methylcatechol | - |
Agaricus bisporus | |
| 0.1 | - |
4-nitrocatechol | - |
Agaricus bisporus | |
| 0.12 | - |
methyl gallate | - |
Agaricus bisporus | |
| 0.15 | - |
Protocatechuic aldehyde | - |
Agaricus bisporus | |
| 0.16 | - |
catechol | - |
Agaricus bisporus | |
| 0.17 | - |
4-Ethylcatechol | - |
Agaricus bisporus | |
| 0.18 | - |
gallic acid | - |
Agaricus bisporus | |
| 0.6 | - |
4-Chlorocatechol | - |
Agaricus bisporus | |
| 0.7 | - |
3,4-Dihydroxyphenylpropionic acid | - |
Agaricus bisporus | |
| 1.3 | - |
3,4-dihydroxyphenylacetic acid | - |
Agaricus bisporus | |
| 1.45 | - |
4-tert-butylcatechol | - |
Agaricus bisporus | |
| 1.96 | - |
pyrogallol | - |
Agaricus bisporus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | - |
Agaricus bisporus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agaricus bisporus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Agaricus bisporus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Agaricus bisporus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8300 | - |
mushroom tyrosinase 8300 U/mg | Agaricus bisporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxyphenylacetic acid + 1/2 O2 | DHPAA | Agaricus bisporus | (3,4-dioxocyclohexa-1,5-dien-1-yl)acetic acid + H2O | - |
? | |
| 3,4-dihydroxyphenylpropionic acid + 1/2 O2 | DHPPA | Agaricus bisporus | 3-(3,4-dioxocyclohexa-1,5-dien-1-yl)propanoic acid + H2O | - |
? | |
| 4-chlorocatechol + 1/2 O2 | - |
Agaricus bisporus | 4-chlorocyclohexa-3,5-diene-1,2-dione + H2O | - |
? | |
| 4-ethylcatechol + 1/2 O2 | - |
Agaricus bisporus | 4-ethylcyclohexa-3,5-diene-1,2-dione + H2O | - |
? | |
| 4-methylcatechol + 1/2 O2 | - |
Agaricus bisporus | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
| 4-nitrocatechol + 1/2 O2 | - |
Agaricus bisporus | 4-nitrocyclohexa-3,5-diene-1,2-dione + H2O | - |
? | |
| 4-tert-butylcatechol + 1/2 O2 | - |
Agaricus bisporus | 4-tert-butylcyclohexa-3,5-diene-1,2-dione + H2O | - |
? | |
| catechol + 1/2 O2 | pyrogallol and catechol are best substrates for catalysis and inactivation | Agaricus bisporus | 1,2-benzoquinone + H2O | - |
? | |
| gallic acid + 1/2 O2 | - |
Agaricus bisporus | 5-hydroxy-3,4-dioxocyclohexa-1,5-diene-1-carboxylic acid + H2O | - |
? | |
| methyl gallate + O2 | - |
Agaricus bisporus | methyl 5-hydroxy-3,4-dioxocyclohexa-1,5-diene-1-carboxylate + H2O | - |
? | |
| protocatechuic acid + 1/2 O2 | - |
Agaricus bisporus | 3,4-dioxocyclohexa-1,5-diene-1-carboxylic acid + H2O | - |
? | |
| protocatechuic aldehyde + 1/2 O2 | - |
Agaricus bisporus | 3,4-dioxocyclohexa-1,5-diene-1-carbaldehyde + H2O | - |
? | |
| pyrogallol + 1/2 O2 | pyrogallol and catechol are best substrates for catalysis and inactivation | Agaricus bisporus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| o-diphenol: oxidoreductase | - |
Agaricus bisporus |
| polyphenol oxidase | - |
Agaricus bisporus |
| tyrosinase | - |
Agaricus bisporus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Agaricus bisporus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
4-nitrocatechol | - |
Agaricus bisporus | |
| 1.5 | - |
Protocatechuic aldehyde | - |
Agaricus bisporus | |
| 8.1 | - |
protocatechuic acid | - |
Agaricus bisporus | |
| 28.2 | - |
gallic acid | - |
Agaricus bisporus | |
| 80.2 | - |
methyl gallate | - |
Agaricus bisporus | |
| 433.1 | - |
3,4-dihydroxyphenylacetic acid | - |
Agaricus bisporus | |
| 607.2 | - |
3,4-Dihydroxyphenylpropionic acid | - |
Agaricus bisporus | |
| 640.1 | - |
4-tert-butylcatechol | - |
Agaricus bisporus | |
| 802.3 | - |
4-Ethylcatechol | - |
Agaricus bisporus | |
| 842.1 | - |
4-methylcatechol | - |
Agaricus bisporus | |
| 859.2 | - |
4-Chlorocatechol | - |
Agaricus bisporus | |
| 874.1 | - |
catechol | - |
Agaricus bisporus | |
| 1280 | - |
pyrogallol | - |
Agaricus bisporus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Agaricus bisporus |
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