Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Dioscorea bulbifera | |
azide | - |
Dioscorea bulbifera | |
CN- | 0.2 mM, 70% inhibition of catechol oxidation, 63% inhibition of pyrogallol oxidation, 50% inhibition of dopa oxidation | Dioscorea bulbifera | |
dithioerythritol | 0.05 mM, 82% inhibition of catechol oxidation, 95% inhibition of pyrogallol oxidation and 62% inhibition of dopa oxidation | Dioscorea bulbifera | |
L-cysteine | 0.5 mM, 61% inhibition of catechol oxidation | Dioscorea bulbifera | |
L-phenylalanine | - |
Dioscorea bulbifera | |
Metabisulfite | - |
Dioscorea bulbifera | |
p-Cresol | - |
Dioscorea bulbifera | |
Thiourea | 0.5 mM, 85% inhibition of catechol oxidation | Dioscorea bulbifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
DL-dopa | - |
Dioscorea bulbifera | |
6.3 | - |
pyrogallol | - |
Dioscorea bulbifera | |
9.1 | - |
catechol | - |
Dioscorea bulbifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
gel filtration | Dioscorea bulbifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dioscorea bulbifera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Dioscorea bulbifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tuber | - |
Dioscorea bulbifera | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
0.0088 units, 1 unit is defined as the amount of enzyme that causes a change in absorbance of 0.001/min | Dioscorea bulbifera |
Storage Stability | Organism |
---|---|
-20°C, acetone powder, long term storage | Dioscorea bulbifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + 1/2 O2 | - |
Dioscorea bulbifera | 1,2-benzoquinone + H2O | - |
? | |
DL-DOPA + O2 | - |
Dioscorea bulbifera | dopaquinone + H2O | - |
r | |
dopa + 1/2 O2 | - |
Dioscorea bulbifera | dopaquinone + H2O | - |
? | |
L-tyrosine + O2 + AH2 | no activity with tyrosine | Dioscorea bulbifera | L-dopa + H2O + A | - |
? | |
p-cresol + O2 | no activity | Dioscorea bulbifera | 4-methylpyrocatechol + H2O | - |
? | |
pyrogallol + O2 | - |
Dioscorea bulbifera | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Dioscorea bulbifera |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
inactivation above | Dioscorea bulbifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Dioscorea bulbifera |