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Literature summary for 1.14.18.1 extracted from

  • S¸derh§ll, K.; Carlberg, I.; Eriksson, T.
    Isolation and partial purification of prophenoloxidase from Daucus carota L. cell cultures (1985), Plant Physiol., 78, 730-733.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Daucus carota
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Nicotiana tabacum
Ca2+
-
Xanthisma gracile
Ca2+ inactive enzyme is maximally activated by 10 mM Ca2+ Daucus carota
copper
-
Daucus carota
Mn2+ activation above 1 mM Mn2+ Daucus carota

Organism

Organism UniProt Comment Textmining
Daucus carota
-
-
-
Nicotiana tabacum
-
tobacco
-
Xanthisma gracile
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, partially purified Nicotiana tabacum
ammonium sulfate, DEAE-cellulose, partially purified Daucus carota
ammonium sulfate, DEAE-cellulose, partially purified Xanthisma gracile

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Daucus carota
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
3314 units, 1 unit is the amount of enzyme causing an increase in absorbance at 490 nm of 0.001/min/ml Daucus carota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4,5-trihydroxybenzoic acid + O2 trivial name gallic acid Daucus carota ?
-
?
4-methylcatechol + O2
-
Nicotiana tabacum 4-methyl-o-benzoquinone + H2O
-
?
4-methylcatechol + O2
-
Daucus carota 4-methyl-o-benzoquinone + H2O
-
?
catechol + 1/2 O2
-
Daucus carota 1,2-benzoquinone + H2O
-
?
chlorogenic acid + O2
-
Daucus carota chlorogenoquinone + H2O
-
?
dopa + 1/2 O2
-
Daucus carota dopaquinone + H2O
-
?