Literature summary for 1.14.18.1 extracted from

Lerch, K.
Monophenol monooxygenase from Neurospora crassa (1987), Methods Enzymol., 142, 165-169.

Search for more literature for 1.14.18.1

Inhibitors

Inhibitors	Comment	Organism	Structure
1-Phenyl-2-thiourea	-	Neurospora crassa
2-mercaptoethanol	-	Neurospora crassa
azide	-	Neurospora crassa
benzhydroxamic acid	-	Neurospora crassa
benzoic acid	-	Neurospora crassa
beta-(N-3-hydroxypyridone-4)-alpha-aminopropionic acid	copper chelator, trivial name L-mimosine	Neurospora crassa
catechol	irreversible inactivation	Neurospora crassa
CO	-	Neurospora crassa
diethyldithiocarbamate	-	Neurospora crassa
L-cysteine	-	Neurospora crassa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
copper	2 gatom per mol	Neurospora crassa
copper	enzyme contains a binuclear copper complex at the active site which is responsible for the interaction with phenolic substrates and the binding and activation of molecular O2, depending on the oxidation state of the copper, 3 different forms are known: Met-, oxy-, and deoxytyrosinase	Neurospora crassa

Organism

Organism	UniProt	Comment	Textmining
Neurospora crassa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfatem CM-cellulose, DEAE-Sephadex, hydroxylapatite	Neurospora crassa

Source Tissue

Source Tissue	Comment	Organism	Textmining
mycelium	-	Neurospora crassa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1205	-	-	Neurospora crassa

Storage Stability

Storage Stability	Organism
-20°C, 10 mM phosphate buffer, pH 7.2, 500 mM NaCl, 30% glycerol, more than 6 months, no loss of activity	Neurospora crassa

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Release 2023.1 (January 2023)