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Literature summary for 1.14.18.1 extracted from

  • Sanchez-Ferrer, A.; Villalba, J.; Garcia-Carmona, F.
    Triton X-114 as a tool for purifying spinach polyphenol oxidase (1989), Phytochemistry, 28, 1321-1325.
No PubMed abstract available
Search for more literature for 1.14.18.1

Activating Compound

Activating Compound Comment Organism Structure
1-decanesulfonic acid slight activation of latent enzyme Spinacia oleracea
additional information activation of latent enzyme under frosty conditions Spinacia oleracea
sodium dodecylsulfate approx. 1.5fold activation of latent enzyme Spinacia oleracea
Trypsin latent enzyme form, 4.5fold activation by trypsin Spinacia oleracea

General Stability

General Stability Organism
inactivation by freezing/thawing Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
4-methylcatechol
-
 Spinacia oleracea
Poly(9)-oxyethylenelauryl ether
-
 Spinacia oleracea
sodium dodecylsulfate above 10 mM inhibition of catecholase activity Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 p-Cresol
-
 Spinacia oleracea
3.3
-
 L-Dopa
-
 Spinacia oleracea
4.2
-
 4-methylcatechol
-
 Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
thylakoid
-
 Spinacia oleracea 9579
-

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2
-
 with 4-methylcatechol Spinacia oleracea
32
-
 with 4-methylcatechol, trypsin activation Spinacia oleracea

Storage Stability

Storage Stability Organism
4°C, achieves full activity in two days without addition of trypsin, 2.5-fold activation at -20 C in 2 months Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcatechol + O2
-
 Spinacia oleracea 4-methyl-o-benzoquinone + H2O
-
 ?
dopa + 1/2 O2
-
 Spinacia oleracea dopaquinone + H2O
-
 ?
p-cresol + O2
-
 Spinacia oleracea 4-methylpyrocatechol + H2O
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
104
-
 4-methylcatechol weak substrate inhibition Spinacia oleracea