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Literature summary for 1.14.18.1 extracted from

  • Oda, Y.; Kato, H.; Isoda, Y.; Takahashi, N.; Yamamoto, T.; Takada, Y.; Kudo, S.
    Purification and properties of phenoloxidase from spinach leaves (1989), Agric. Biol. Chem., 53, 2053-2061.
No PubMed abstract available
Search for more literature for 1.14.18.1

Inhibitors

Inhibitors Comment Organism Structure
Acetylacetone 0.1 mM, 75% inhibition Spinacia oleracea
Bathocuproine
-
 Spinacia oleracea
Cuprizone
-
 Spinacia oleracea
Cuproine
-
 Spinacia oleracea
diethyldithiocarbamate 0.1 mM, 80% inhibition Spinacia oleracea
DL-dithiothreitol
-
 Spinacia oleracea
EDTA no inhibition EDTA Spinacia oleracea
additional information not inhibited by 2,2'-dipyridyl Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.87
-
 Protocatechuic aldehyde
-
 Spinacia oleracea
3.24
-
 esculetin
-
 Spinacia oleracea
4.75
-
 4-methylcatechol
-
 Spinacia oleracea
5.15
-
 chlorogenic acid
-
 Spinacia oleracea
6.43
-
 caffeic acid
-
 Spinacia oleracea
7.95
-
 catechol
-
 Spinacia oleracea
8.63
-
 Dopa
-
 Spinacia oleracea
27.4
-
 Hydrocaffeic acid
-
 Spinacia oleracea
189
-
 protocatechuic acid
-
 Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
copper 2 atoms of copper per 150000 Da Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 4 * 40000, SDS-PAGE Spinacia oleracea
150000
-
 gel filtration Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
salting out and Sephadex G-75, DEAE-cellulose, DEAE-Sephadex A-25, hydroxylapatite Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.277
-
 with catechol Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcatechol + O2
-
 Spinacia oleracea 4-methyl-o-benzoquinone + H2O
-
 ?
4-nitrocatechol + O2
-
 Spinacia oleracea ?
-
 ?
caffeic acid + 1/2 O2
-
 Spinacia oleracea caffeoyl quinone + H2O
-
 ?
catechol + 1/2 O2
-
 Spinacia oleracea 1,2-benzoquinone + H2O
-
 ?
chlorogenic acid + O2
-
 Spinacia oleracea chlorogenoquinone + H2O
-
 ?
dopa + 1/2 O2
-
 Spinacia oleracea dopaquinone + H2O
-
 ?
esculetin + O2
-
 Spinacia oleracea ?
-
 ?
hydrocaffeic acid + O2
-
 Spinacia oleracea ?
-
 ?
protocatechuic acid + O2
-
 Spinacia oleracea ?
-
 ?
protocatechuic aldehyde + O2
-
 Spinacia oleracea ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Spinacia oleracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
123
-
 esculetin
-
 Spinacia oleracea
336
-
 Protocatechuic aldehyde
-
 Spinacia oleracea
860
-
 protocatechuic acid
-
 Spinacia oleracea
1450
-
 caffeic acid
-
 Spinacia oleracea
1650
-
 chlorogenic acid
-
 Spinacia oleracea
2960
-
 catechol
-
 Spinacia oleracea
4630
-
 Hydrocaffeic acid
-
 Spinacia oleracea
4940
-
 Dopa
-
 Spinacia oleracea
7220
-
 4-methylcatechol
-
 Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Spinacia oleracea

pH Range

pH Minimum pH Maximum Comment Organism
5 8 high activity in the range pH 5-8 Spinacia oleracea