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Literature summary for 1.14.17.4 extracted from

  • Binnie, J.E.; McManus, M.T.
    Characterization of the 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase multigene family of Malus domestica Borkh (2009), Phytochemistry, 70, 348-360.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pGEM-T Easy and subsequently into pProEX-1 for expression of the protein in Escherichia coli BL21 cells Malus domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08939
-
1-aminocyclopropane-1-carboxylic acid
-
Malus domestica
0.2445
-
1-aminocyclopropane-1-carboxylic acid
-
Malus domestica
0.401
-
1-aminocyclopropane-1-carboxylic acid
-
Malus domestica

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Malus domestica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35350
-
determined by SDA-PAGE and Western blot analysis Malus domestica
36310
-
determined by SDA-PAGE and Western blot analysis Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-aminocyclopropane-1-carboxylic acid + ascorbate + O2 Malus domestica
-
ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Malus domestica
-
-
-
Malus domestica O48882
-
-
Malus domestica Q00985
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant ACO is purified by affinity chromatography using nickel nitrilotriacetic acid resin, furthermore Sephadex G-25 resin and a Mono-Q column are used, in addition ACO is extracted from apple leaf and fruit tissue Malus domestica

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Malus domestica
-
leaf
-
Malus domestica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-aminocyclopropane-1-carboxylic acid + ascorbate + O2
-
Malus domestica ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
?

Synonyms

Synonyms Comment Organism
1-aminocyclopropane-1-carboxylic acid oxidase
-
Malus domestica
ACC oxidase
-
Malus domestica
ACO
-
Malus domestica
MD-ACO1
-
Malus domestica
MD-ACO2
-
Malus domestica
MD-ACO3
-
Malus domestica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0344
-
1-aminocyclopropane-1-carboxylic acid
-
Malus domestica
0.066
-
1-aminocyclopropane-1-carboxylic acid
-
Malus domestica
0.0914
-
1-aminocyclopropane-1-carboxylic acid
-
Malus domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Malus domestica
7.5
-
-
Malus domestica
7.8
-
-
Malus domestica

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5
-
Malus domestica
6.5 8
-
Malus domestica