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Literature summary for 1.14.17.3 extracted from

  • Bauman, A.T.; Ralle, M.; Blackburn, N.J.
    Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor (2007), Protein Expr. Purif., 51, 34-38.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in CHO DG44 cells Rattus norvegicus
expression of the truncated enzyme, comprising the catalytic core residues 42-356, in CHO DG44 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information only the residues 42-356 carrying the monoogenase domain was expressed Rattus norvegicus
additional information large scale production of the enzyme using an automated bioreactor, method optimization and evaluation, overview Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a copper enzyme, copper binding ratios of recombinant catalytic core, overview Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35040
-
MALDI-MS, recombinant enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
large scale purification of the recombinant truncated enzyme, comprising the catalytic core residues 42-356, from CHO DG44 cells, reconstitution of copper bound to the enzyme Rattus norvegicus
two chromatoography steps Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl-D-Tyr-Val-Gly + ascorbate + O2
-
Rattus norvegicus dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
?

Synonyms

Synonyms Comment Organism
PAM
-
Rattus norvegicus
peptidylglycine alpha-hydroxylating monooxygenase
-
Rattus norvegicus
peptidylglycine monooxygenase
-
Rattus norvegicus
PHM
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus