Literature summary for 1.14.17.3 extracted from

Francisco, W.A.; Merkler, D.J.; Blackburn, N.J.; Klinman, J.P.
Kinetic mechanism and intrinsic isotope effects for the peptidylglycine alpha-amidating enzyme reaction (1998), Biochemistry, 37, 8244-8252.

Search for more literature for 1.14.17.3

Cloned(Commentary)

Cloned (Comment)	Organism
medullary thyroid alpha-AE, type A, expression in chinese hamster ovary cells	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	recombinant enzyme secreted into medium	Rattus norvegicus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	required for alpha-hydroxylation step	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
peptidylglycine + ascorbate + O2	Rattus norvegicus	-	peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	bifunctional alpha-AE	-

Purification (Commentary)

Purification (Comment)	Organism
type A alpha-AE, recombinant from chinese hamster ovary cells	Rattus norvegicus

Reaction

Reaction	Comment	Organism	Reaction ID
[peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O	sequential activity of 2 enzymes contained within a bifunctional protein	Rattus norvegicus	a
[peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O	equilibrium ordered mechanism	Rattus norvegicus	a
[peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O	kinetic analysis	Rattus norvegicus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
medullary thyroid carcinoma cell	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hippuric acid + ascorbate + O2	i.e. N-benzoylglycine	Rattus norvegicus	alpha-hydroxyhippuric acid + dehydroascorbate + H2O	-	?
peptidylglycine + ascorbate + O2	-	Rattus norvegicus	peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O	-	?
peptidylglycine + ascorbate + O2	requirement for O2	Rattus norvegicus	peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O	-	?
peptidylglycine + ascorbate + O2	COOH-terminal glycine	Rattus norvegicus	peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
More	the 2-step process is catalyzed by only 1 enzyme called type A peptidylglycine alpha-amidating enzyme	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
ascorbate	dependent on	Rattus norvegicus

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Release 2023.1 (January 2023)