Literature summary for 1.14.17.3 extracted from

Bell, J.; Ash, D.E.; Snyder, L.M.; Kulathila, R.; Blackburn, N.J.; Merkler, D.J.
Structural and functional investigations on the role of zinc in bifunctional rat peptidylglycine alpha-amidating enzyme (1997), Biochemistry, 36, 16239-16246.

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Cloned(Commentary)

Cloned (Comment)	Organism
medullary thyroid alpha-AE, type A, expression in chinese hamster ovary cells	Rattus norvegicus

General Stability

General Stability	Organism
Cu2+ can restore EDTA-treated only the alpha-hydroxylating activity	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	recombinant enzyme, alpha-hydroxylation activity can be restored by Mn2+, Zn2+, Cd2+, and Co2+, but not by Ca2+, Cu2+, Mg2+, and Fe3+	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	recombinant enzyme secreted into medium	Rattus norvegicus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	required for alpha-hydroxylation step	Rattus norvegicus
Zn2+	required for alpha-amidation step	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
peptidylglycine + ascorbate + O2	Rattus norvegicus	-	peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P14925	bifunctional alpha-AE	-

Purification (Commentary)

Purification (Comment)	Organism
type A alpha-AE, recombinant from chinese hamster ovary cells	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
medullary thyroid carcinoma cell	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dansyl-D-Tyr-Val-Gly + ascorbate + O2	-	Rattus norvegicus	dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O	-	?
N-dansyl-Tyr-Val-Gly + ascorbate + O2	-	Rattus norvegicus	(S)-N-dansyl-Tyr-Val-alpha-hydroxyglycine + dehydroascorbate + H2O	-	ir
peptidylglycine + ascorbate + O2	-	Rattus norvegicus	peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O	-	?
peptidylglycine + ascorbate + O2	COOH-terminal glycine	Rattus norvegicus	peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
alpha-AE	peptidylglycine alpha-amidating enzyme	Rattus norvegicus
More	the 2-step process is catalyzed by only 1 enzyme called type A peptidylglycine alpha-amidating enzyme	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
ascorbate	dependent on	Rattus norvegicus

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Release 2023.1 (January 2023)