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Literature summary for 1.14.17.3 extracted from

  • Tajima, M.; Iida, T.; Yoshida, S.; Komatsu, K.; Namba, R.; Yanagi, M.; Noguchi, M.; Okamoto, H.
    The reaction product of peptidylglycine alpha-amidating enzyme is a hydroxyl derivative at alpha-carbon of the carboxyl-terminal glycine (1990), J. Biol. Chem., 265, 9602-9605.
    View publication on PubMed
Search for more literature for 1.14.17.3

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ Cu2+ is absolutely required for optimal activity Equus caballus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidylglycine + ascorbate + O2 Equus caballus
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Equus caballus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Equus caballus

Reaction

Reaction Comment Organism Reaction ID
[peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O structure analysis Equus caballus
a

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Equus caballus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information at alkaline pH spontaneous conversion Equus caballus ?
-
 ?
additional information EC 1.14.17.3 is often called peptidylglycine alpha-amidating monooxygenase (PAM) and the alpha-amidated product is mentioned as the product of the reaction, but the alpha-amidation of glycine-extended peptides is a two-step process catalyzed by 2 enzymes: 1. EC 1.14.17.3: production of peptidyl(2-hydroxyglycine) by a copper, molecular oxygen and ascorbate-dependent peptidyl-glycine alpha-hydroxylating monooxygenase (PMH) and 2. conversion of the peptidyl-alpha-hydroxyglycine derivative into an alpha-amidated product at physiological pH by peptidyl-alpha-hydroxyglycine alpha-amidating lyase Equus caballus ?
-
 ?
peptidylglycine + ascorbate + O2
-
 Equus caballus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
peptidylglycine + ascorbate + O2 requirement for O2 Equus caballus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
peptidylglycine + ascorbate + O2 COOH-terminal glycine Equus caballus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
Phe-Gly-Phe-Gly + ascorbate + O2
-
 Equus caballus Phe-Gly-Phe-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
More at alkaline pH spontaneous conversion Equus caballus
More EC 1.14.17.3 is often called peptidylglycine alpha-amidating monooxygenase (PAM) and the alpha-amidated product is mentioned as the product of the reaction, but the alpha-amidation of glycine-extended peptides is a two-step process catalyzed by 2 enzymes: 1. EC 1.14.17.3: production of peptidyl(2-hydroxyglycine) by a copper, molecular oxygen and ascorbate-dependent peptidyl-glycine alpha-hydroxylating monooxygenase (PHM), 2. conversion of the peptidyl-alpha-hydroxyglycine derivative into an alpha-amidated product at physiological pH by peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PHL) Equus caballus

Cofactor

Cofactor Comment Organism Structure
ascorbate dependent on Equus caballus