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Literature summary for 1.14.17.3 extracted from

  • Beaudry, G.A.; Mehta, N.M.; Ray, M.I.; Bertelsen, A.H.
    Purification and characterization of functional recombinant alpha-amidating enzyme secreted from mammalian cells (1990), J. Biol. Chem., 265, 17694-17699.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
medullary thyroid carcinoma enzyme, expression in mouse C 127 cells via bovine papilloma virus vector, amino acid and DNA sequence analysis Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
dansyl-Tyr-Val-Gly recombinant enzyme Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
recombinant from mouse cells, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidylglycine + ascorbate + O2 Rattus norvegicus
-
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein partially glycosylated, at Asn 660, recombinant enzyme from mouse cells Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
recombinant from mouse cells Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
pituitary gland
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15
-
recombinant purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl-D-Tyr-Val-Gly + ascorbate + O2
-
Rattus norvegicus dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
?
peptidylglycine + ascorbate + O2
-
Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?
peptidylglycine + ascorbate + O2 COOH-terminal glycine Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 75000, recombinant enzyme, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5 recombinant enzyme Rattus norvegicus
7
-
assay at, recombinant enzyme Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
4 9 recombinant enzyme Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
rapid inactivation of recombinant enzyme at acidic pH in vitro Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ascorbate dependent on Rattus norvegicus