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Literature summary for 1.14.17.3 extracted from
- Enzyme-catalysed peptide amidation. Isolation of a stable intermediate formed by reaction of the amidating enzyme with an imino acid (1987), Eur. J. Biochem., 169, 579-584.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-Tyr-Val-Gly | at 2 mM 82% inhibition, at 0.1 mM 45% inhibition | Sus scrofa |  |
| Glyoxylate 2-pyridylhydrazone | - |
Sus scrofa | |
| Glyoxylate 4-carboxyphenylhydrazone | - |
Sus scrofa | |
| Glyoxylate benzylhydrazone | - |
Sus scrofa | |
| glyoxylate phenylhydrazone | strongly | Sus scrofa | |
| Glyoxylate phenylsemicarbazone | - |
Sus scrofa | |
| Glyoxylate phenylthiosemicarbazone | - |
Sus scrofa | |
| Glyoxylate semicarbazone | - |
Sus scrofa | |
| glyoxylate thiosemicarbazone | - |
Sus scrofa | |
| glyoxylate-N-hexyl-4-carbamoylphenylhydrazone | strongly | Sus scrofa | |
| N-Carboxymethyl N'-phenylhydrazone | weak inhibition | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel fltration, SDS-PAGE | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptidylglycine + ascorbate + O2 | Sus scrofa | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| [peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O | mechanism | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pituitary gland | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Tyr-Val-Gly + ascorbate + O2 | - |
Sus scrofa | D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
| glyoxylic acid phenylhydrazone + ascorbate + O2 | competitive substrate, inhibitory | Sus scrofa | oxalate phenylhydrazide + dehydroascorbate + H2O | - |
? | |
| additional information | substrates are also physiologically relevant peptides related to alpha-melanotropine | Sus scrofa | ? | - |
? | |
| peptidylglycine + ascorbate + O2 | - |
Sus scrofa | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
| peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Sus scrofa | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Sus scrofa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | dependent on | Sus scrofa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
glyoxylate phenylhydrazone | - |
Sus scrofa | |
| 0.11 | - |
glyoxylate thiosemicarbazone | - |
Sus scrofa | |
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Release 2023.1 (January 2023)
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