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Literature summary for 1.14.17.3 extracted from

  • Glembotski, C.C.
    Further characterization of the peptidyl alpha-amidating enzyme in rat anterior pituitary secretory granules (1985), Arch. Biochem. Biophys., 241, 673-683.
    View publication on PubMed
Search for more literature for 1.14.17.3

General Stability

General Stability Organism
repeated freeze-thaw cycles have no effect on enzyme activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ascorbate above 0.4 mM for the purified enzyme, above 1.5 mM for the enzyme in crude extract Rattus norvegicus
Cu2+ at higher concentration Rattus norvegicus
diethyldithiocarbamate copper chelator Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for D-Tyr-Val-Gly depends on concentration of ascorbate, Km for ascorbate is constant over a wide range of D-Tyr-Val-Gly concentration Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
secretory granule
-
 Rattus norvegicus 30141
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a copper protein Rattus norvegicus
Cu2+ Cu2+ is absolutely required for optimal activity Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidylglycine + ascorbate + O2 Rattus norvegicus
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
pituitary gland anterior, intermediate and posterior Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adrenocorticotrophic hormone(9-14) + ascorbate + O2
-
 Rattus norvegicus adrenocorticotrophic hormone(9-13)-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
alpha-N-acetyl-adrenocorticotrophic hormone(1-14) + ascorbate + O2
-
 Rattus norvegicus alpha-N-acetyl-adrenocorticotrophic hormone(1-13)-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
D-Tyr-Val-Gly + ascorbate + O2
-
 Rattus norvegicus D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
additional information substrates are also physiologically relevant peptides related to alpha-melanotropine Rattus norvegicus ?
-
 ?
peptidylglycine + ascorbate + O2
-
 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
peptidylglycine + ascorbate + O2 COOH-terminal glycine Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 incubation for 12-15 h, 47% loss of activity Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ascorbate dependent on Rattus norvegicus