Cloned (Comment) | Organism |
---|---|
expressed in CHO-K1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C231A | the mutant enzyme activity is not significantly different from wild type | Homo sapiens |
C397A | the mutant enzyme activity is not significantly different from wild type | Homo sapiens |
D153A | the mutant enzyme activity is not significantly different from wild type | Homo sapiens |
D384A | the mutant has significantly reduced, but clearly detectable, enzyme activity | Homo sapiens |
E137A | the mutation leads to an 18fold increase in the Michaelis-Menten constant for tetrahydrobiopterin. The mutant has an approximately 5fold lower affinity for iron | Homo sapiens |
E152A | the mutant has significantly reduced, but clearly detectable, enzyme activity | Homo sapiens |
E203A | the mutant has significantly reduced, but clearly detectable, enzyme activity | Homo sapiens |
E212A | the mutant has significantly reduced, but clearly detectable, enzyme activity | Homo sapiens |
H189A | the mutant has significantly reduced, but clearly detectable, enzyme activity | Homo sapiens |
H220A | the mutant enzyme activity is not significantly different from wild type | Homo sapiens |
Q146A | the mutant enzyme activity is not significantly different from wild type | Homo sapiens |
Q166A | the mutant has significantly reduced, but clearly detectable, enzyme activity | Homo sapiens |
Q191A | the mutant has significantly reduced, but clearly detectable, enzyme activity | Homo sapiens |
Q197A | the mutant has activity of less than 5% of that of the wild type | Homo sapiens |
R396A | the mutant has significantly reduced, but clearly detectable, enzyme activity | Homo sapiens |
Y174A | the mutant has significantly reduced, but clearly detectable, enzyme activity | Homo sapiens |
Y230A | the mutant enzyme activity is not significantly different from wild type | Homo sapiens |
Y338A | the mutant has activity of less than 5% of that of the wild type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phenanthroline | competitive inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00183 | - |
tetrahydrobiopterin | mutant enzyme E137A, at pH 8.5 and 37°C | Homo sapiens | |
0.0335 | - |
tetrahydrobiopterin | wild type enzyme, at pH 8.5 and 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-pyrenedecyl-sn-glycerol + tetrahydrobiopterin + O2 | - |
Homo sapiens | ? + dihydrobiopterin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
alkylglycerol mono-oxygenase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | dependent on | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00069 | - |
wild type enzyme, at pH 8.5 and 37°C | Homo sapiens | Phenanthroline | |
0.00341 | - |
mutant enzyme E137A, at pH 8.5 and 37°C | Homo sapiens | Phenanthroline |