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Literature summary for 1.14.16.4 extracted from
- Zebrafish tyrosine hydroxylase 2 gene encodes tryptophan hydroxylase (2013), J. Biol. Chem., 288, 22451-22459.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Danio rerio |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
x * 90000, SDS-PAGE | Danio rerio |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tryptophan + tetrahydrobiopterin + O2 | Danio rerio | - |
5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Danio rerio | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione-Sepharose column chromatography, and gel filtration | Danio rerio |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Danio rerio | - |
| diencephalon | - |
Danio rerio | - |
| hypothalamus | - |
Danio rerio | - |
| neuron | - |
Danio rerio | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0144 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Danio rerio |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tryptophan + tetrahydrobiopterin + O2 | - |
Danio rerio | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? | |
| additional information | no activity with L-tyrosine | Danio rerio | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90000, SDS-PAGE | Danio rerio |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TH2 | - |
Danio rerio |
| TPH | - |
Danio rerio |
| tryptophan hydroxylase | - |
Danio rerio |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| tetrahydrobiopterin | - |
Danio rerio | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the th2 gene product is required for the synthesis of serotonin in vivo and has tryptophan hydroxylase activity in vitro | Danio rerio |
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Release 2023.1 (January 2023)
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