Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type isozyme TPH1 in COS7 cells | Homo sapiens |
expression of wild-type isozyme TPH2, and of TPH2 deletion mutants in COS7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of various deletion mutants of TPH2: DN44, DC17, DC19,and DC51. Removal of 44 amino acids from the N-terminus of TPH2 results in a 3-4fold increased Vmax, which indicates a strong inhibitory function of this part on the enzymes activity. Deletion mutants DC17, DC19and DC51, which lack the putative C-terminal leucine zipper tetramerization domain, exist as monomeric enzymes | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | removal of 44 amino acids from the N-terminus of TPH2 results in a 3-4fold increased Vmax, which indicates a strong inhibitory function of this part on the enzymes activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type isozyme TPH1, overview | Homo sapiens | |
additional information | - |
additional information | kinetics of wild-type isozyme TPH2 and of TPH2 deletion mutants, overview | Homo sapiens | |
0.015 | - |
tetrahydrobiopterin | pH 7.0, 30°C, recombinant isozyme TPH1 | Homo sapiens | |
0.053 | - |
6-methyl-tetrahydropterin | pH 7.0, 30°C, recombinant isozyme TPH1 | Homo sapiens | |
0.077 | - |
tetrahydrobiopterin | pH 7.0, 30°C, recombinant isozyme TPH2 | Homo sapiens | |
0.802 | - |
6-methyl-tetrahydropterin | pH 7.0, 30°C, recombinant isozyme TPH2 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + tetrahydrobiopterin + O2 | Homo sapiens | first and rate limiting step in the biosynthesis of the neurotransmitter serotonin | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme TPH1 | - |
Homo sapiens | - |
isozyme TPH2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + 6-methyl-tetrahydropterin + O2 | - |
Homo sapiens | 5-hydroxy-L-tryptophan + 6-methyl-4a-hydroxytetrahydropterin | - |
? | |
L-tryptophan + tetrahydrobiopterin + O2 | - |
Homo sapiens | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-tryptophan + tetrahydrobiopterin + O2 | first and rate limiting step in the biosynthesis of the neurotransmitter serotonin | Homo sapiens | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TPH1 | - |
Homo sapiens |
TPH2 | - |
Homo sapiens |
tryptophan hydroxylase 1 | - |
Homo sapiens |
tryptophan hydroxylase 2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |