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Literature summary for 1.14.16.4 extracted from

  • Tong, J.H.; Kaufman, S.
    Tryptophan hydroxylase. Purification and some properties of the enzyme from rabbit hindbrain (1975), J. Biol. Chem., 250, 4152-4158.
    View publication on PubMed
Search for more literature for 1.14.16.4

Inhibitors

Inhibitors Comment Organism Structure
L-phenylalanine substrate inhibition with tetrahydrobiopterin as cofactor, above 0.5 mM Oryctolagus cuniculus
L-tryptophan substrate inhibition with 2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine, not with 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine, above 0.2 mM Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 L-tryptophan cofactor tetrahydrobiopterin Oryctolagus cuniculus
0.286
-
 phenylalanine cofactor tetrahydrobiopterin Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57700
-
 2 * 57700 + 2 * 60900, hindbrain enzyme, SDS-PAGE Oryctolagus cuniculus
60900
-
 2 * 57700 + 2 * 60900, hindbrain enzyme, SDS-PAGE Oryctolagus cuniculus
230000
-
 gel filtration, PAGE, sucrose density gradient centrifugation Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information less than 1% bound carbohydrate Oryctolagus cuniculus

Purification (Commentary)

Purification (Comment) Organism
hindbrain enzyme, calcium phosphate gel, polyethylene glycol 6000, Sepharose 6B, DEAE-cellulose Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
brain hindbrain Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0021
-
 hindbrain enzyme Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydropteridine + O2 28.8% of the rate observed with L-tryptophan Oryctolagus cuniculus tyrosine + dihydropteridine + H2O
-
 ?
L-tryptophan + tetrahydropteridine + O2 cofactor tetrahydrobiopterin Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?
L-tryptophan + tetrahydropteridine + O2 cofactor 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?
L-tryptophan + tetrahydropteridine + O2 cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 57700 + 2 * 60900, hindbrain enzyme, SDS-PAGE Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.197
-
 L-phenylalanine
-
 Oryctolagus cuniculus