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Literature summary for 1.14.16.4 extracted from
- Tryptophan 5-monooxygenase from mouse mastocytoma: high-performance liquid chromatography assay (1987), Methods Enzymol., 142, 88-92.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | essential for activation | Mus musculus |  |
| dithiothreitol | - |
Mus musculus | |
General Stability
| General Stability | Organism |
|---|---|
| catalase at a concentration of more than 1 mg/ml is required for stability during reaction at aerobic conditiones | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | 0.033 | L-tryptophan | activated enzyme in desalted extracts | Mus musculus | |
| 0.294 | - |
tetrahydrobiopterin | activated enzyme in desalted extracts | Mus musculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mastocytoma cell | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tryptophan + tetrahydropteridine + O2 | - |
Mus musculus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
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