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Literature summary for 1.14.16.2 extracted from
- Cloning and characterization of a novel enzyme: tyrosine hydroxylase from Schistosoma japonicum (2011), Parasitol. Res., 109, 1065-1074.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| a His-tagged version expressed in Escherichia coli BL21 (DE3) | Schistosoma japonicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-tyrosine | substrate inhibition at concentrations above 0.05 mM | Schistosoma japonicum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0426 | - |
tetrahydrobiopterin | pH 6.9, 37°C | Schistosoma japonicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, predicted, 55 kDa from SDS-PAGE of His-tagged version | Schistosoma japonicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schistosoma japonicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography (Ni2+) | Schistosoma japonicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.82 | - |
pH 6.9, 37°C | Schistosoma japonicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine + tetrahydrobiopterin + O2 | - |
Schistosoma japonicum | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, predicted, 55 kDa from SDS-PAGE of His-tagged version | Schistosoma japonicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tyrosine hydroxylase | - |
Schistosoma japonicum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Schistosoma japonicum | predicted | - |
5.81 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Schistosoma japonicum | in cercaria compared with the adult worm | down |
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Release 2023.1 (January 2023)
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