Activating Compound | Comment | Organism | Structure |
---|---|---|---|
forskolin | forskolin selectively increases the phosphorylation of tyrosine hydroxylase at Ser40 by 1.8fold without increasing total tyrosine hydroxylase protein levels | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the N-terminus of tyrosine hydroxylase removes the high affinity dopamine binding site, but does not affect dopamine binding to the low affinity site | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dihydroxy-L-phenylalanine | incubation with 3,4-dihydroxy-L-phenylalanine results in an inhibition of tyrosine hydroxylase activity in situ under both basal conditions and conditions that promote the phosphorylation of Ser40. 3,4-dihydroxy-L-phenylalanine binding to the high affinity site alone inhibits tyrosine hydroxylase activity to approximately 15% and 24% of control (non-dopamine bound) levels at 0.02 mM and 2.0 mM 5,6,7,8-tetrahydrobiopterin, respectively | Homo sapiens | |
additional information | there is no significant decrease in tyrosine hydroxylase activity at 0.02 mM or 2.0 mM 5,6,7,8-tetrahydrobiopterin following ovalbumin-coated charcoal treatment | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-chelating Sepharose fast flow bead chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + 5,6,7,8,-tetrahydro-L-biopterin + O2 | - |
Homo sapiens | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydro-L-biopterin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TH1 | isozyme | Homo sapiens |
tyrosine hydroxylase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | tyrosine hydroxylase is the rate-limiting enzyme in catecholamine biosynthesis | Homo sapiens |