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Literature summary for 1.14.16.2 extracted from

  • Jin, C.M.; Yang, Y.J.; Huang, H.S.; Lim, S.C.; Kai, M.; Lee, M.K.
    Induction of dopamine biosynthesis by l-DOPA in PC12 cells: implications of L-DOPA influx and cyclic AMP (2008), Eur. J. Pharmacol., 591, 88-95.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
L-Dopa L-DOPA treatment (20-200 microM) increases the levels of dopamine by 226%-504% after 3-6 h of treatment and enhances the activities of tyrosine hydroxylase and aromatic L-amino acid decarboxylase Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
L-Dopa Increased levels of intracellular L-DOPA inhibits tyrosine hydroxylase activity by end-product inhibition at early time points, L-DOPA (20-200 microM) treatment leads to a 562%-937% increase in L-DOPA influx at 1 h, which inhibits the activity of tyrosine hydroylase during the same period. Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + tetrahydropteridine + O2 Rattus norvegicus
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3,4-dihydroxyphenylalanine + dihydropteridine + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
PC-12 cell derived from a pheochromocytoma of the rat adrenal medulla Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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217% activity compared to control level, treatment with 0.02 mM L-DOPA in PC-12 cells at 6 h time point Rattus norvegicus
additional information
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232% activity compared to control level, treatment with 0.1 mM L-DOPA in PC-12 cells at 6 h time point Rattus norvegicus
additional information
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272% activity compared to control level, treatment with 0.02 mM L-DOPA in PC-12 cells at 6 h time point Rattus norvegicus
additional information
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55.2% activity compared to control level, treatment with 0.2 mM L-DOPA in PC-12 cells at 48 h time point Rattus norvegicus
additional information
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62.6% activity compared to control level, treatment with 0.2 mM L-DOPA in PC-12 cells at 1 h time point Rattus norvegicus
additional information
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68.2% activity compared to control level, treatment with 0.1 mM L-DOPA in PC-12 cells at 1 h time point Rattus norvegicus
additional information
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82.2% activity of tyrosine hydroxylase compared to control level, treatment with 0.02 mM L-DOPA in PC-12 cells at 1 h time point Rattus norvegicus
additional information
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82.3% activity compared to control level, treatment with 0.1 mM L-DOPA in PC-12 cells at 48 h time point Rattus norvegicus
additional information
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activity returns to the control level between 24-48 h after treatment with 0.02 mM L-DOPA. Rattus norvegicus
0.00368
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activity of tyrosine hydroxylase, control level without treatment of L-DOPA in PC-12 cells Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + tetrahydropteridine + O2
-
Rattus norvegicus 3,4-dihydroxyphenylalanine + dihydropteridine + H2O
-
?

Synonyms

Synonyms Comment Organism
tyrosine hydroxylase
-
Rattus norvegicus