Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes H331E, H336Q, H336E, E376Q, E376H, H331E/E376H in Escherichia coli BL21 | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E376H | iron content is not significantly altered. Pterin oxidation at 1.2% of the wild-type activity. Tyrosine hydroxylation is less than 0.4% of the wild-type value | Rattus norvegicus |
E376Q | iron content is not significantly altered. Pterin oxidation at 0.4% of the wild-type activity. Tyrosine hydroxylation is 0.39% of the wild-type value | Rattus norvegicus |
H331E | iron content is not significantly altered | Rattus norvegicus |
H331E/E376H | mutant enzyme contains significantly less iron than the wild-type enzyme. Pterin oxidation at 0.21% of the wild-type activity. Tyrosine hydroxylation is less than 0.4% of the wild-type value | Rattus norvegicus |
H331Q | mutant enzyme is not successfully expressed. Pterin oxidation at 2.4% of the wild-type activity. Tyrosine hydroxylation is less than 0.002% of the wild-type value | Rattus norvegicus |
H336E | significant decrease in iron content. Pterin oxidation at 6.3% of the wild-type activity. Tyrosine hydroxylation is 0.78% of the wild-type value | Rattus norvegicus |
H336Q | iron-free mutant enzyme. Pterin oxidation at 11.9% of the wild-type activity. Tyrosine hydroxylation is 3.7% of the wild-type value | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.051 | - |
L-Tyr | pH 7.1, 25°C, wild-type enzyme | Rattus norvegicus | |
0.051 | - |
6-methyltetrahydropterin | pH 7.1, 25°C, wild-type enzyme | Rattus norvegicus | |
0.19 | - |
6-methyltetrahydropterin | pH 7.1, 25°C, mutant enzyme H336E | Rattus norvegicus | |
0.311 | - |
L-Tyr | pH 7.1, 25°C, mutant enzyme H336Q | Rattus norvegicus | |
0.33 | - |
L-Tyr | pH 7.1, 25°C, mutant enzyme H336E | Rattus norvegicus | |
0.339 | - |
6-methyltetrahydropterin | pH 7.1, 25°C, mutant enzyme H331E | Rattus norvegicus | |
0.409 | - |
6-methyltetrahydropterin | pH 7.1, 25°C, mutant enzyme H336Q | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + 6-methyltetrahydropterin + O2 | - |
Rattus norvegicus | ? | - |
? |