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Literature summary for 1.14.16.2 extracted from

  • Daubner, S.C.; Melendez, J.; Fitzpatrick, P.F.
    Reversing the substrate specificities of phenylalanine and tyrosine hydroxylase: aspartate 425 of tyrosine hydroxylase is essential for L-DOPA formation (2000), Biochemistry, 39, 9652-9661.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D425V 335fold reduced tyrosine hydroxylation/dopa formation activity, 120fold reduced reaction velocity with tyrosine, 3fold enhanced phenylalanine hydroxylation activity, active site mutant Rattus norvegicus
H323Y enhanced Km for tyrosine, 4.5fold enhanced phenylalanine hydroxylation activity, active site mutant Rattus norvegicus
additional information active site mutants of phenylalanine hydroxylase lead to highly increased tyrosine hydroxylation activity of the enzyme mutants Rattus norvegicus
Q310H 4fold reduced tyrosine hydroxylation/dopa formation activity, slightly enhanced phenylalanine hydroxylation activity, active site mutant Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-values for tyrosine of mutant phenylalanine hydroxylase with tyrosine hydroxylation activity Rattus norvegicus
0.00125
-
phenylalanine recombinant mutant D425V Rattus norvegicus
0.0077
-
phenylalanine recombinant mutant H323Y Rattus norvegicus
0.0088
-
phenylalanine recombinant mutant Q310H Rattus norvegicus
0.016
-
tyrosine recombinant wild-type Rattus norvegicus
0.045
-
tyrosine recombinant mutant D425V Rattus norvegicus
0.054
-
tyrosine recombinant mutant Q310H Rattus norvegicus
0.092
-
tyrosine recombinant mutant H323Y Rattus norvegicus
0.1
-
phenylalanine recombinant wild-type Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P04177
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutants from Escherichia coli Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydropteridine + 2 O2 wild-type and mutants Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydropteridine + 2 H2O
-
?
L-tyrosine + tetrahydrobiopterin + O2
-
Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?

Synonyms

Synonyms Comment Organism
TH
-
Rattus norvegicus