Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
3D-structure model, ligand binding of pterin analogues | Rattus norvegicus |
crystals of the binary complex with iron and 7,8-dihydrobiopterin obtained by equilibrium dialysis, solutions degassed by helium and crystal growth in nitrogen atmosphere at 4°C | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | investigation of the role of several amino acid residues in binding of substrate and ligands by site-specific mutagenesis | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-[(3-azido-6-nitrobenzylidene)amino]-2,6-diamino-4-pyrimidinone | competitive against tetrahydrobiopterin | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-tyrosine + a 5,6,7,8-tetrahydropteridine + O2 = L-dopa + a 4a-hydroxy-5,6,7,8-tetrahydropteridine | stereochemical analysis of ligand binding | Rattus norvegicus | |
L-tyrosine + a 5,6,7,8-tetrahydropteridine + O2 = L-dopa + a 4a-hydroxy-5,6,7,8-tetrahydropteridine | active binding site | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + 6-methyltetrahydropterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? |