Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Dextran sulfate | activates the enzyme in the crude extract, less effective with the purified enzyme | Rattus norvegicus | |
heparin | activates the enzyme in the crude extract, less effective with the purified enzyme | Rattus norvegicus | |
phosphatidyl-L-serine | no stimulation | Rattus norvegicus | |
phosphatidylinositol | no stimulation | Rattus norvegicus | |
Phospholipid | no stimulation | Rattus norvegicus |
General Stability | Organism |
---|---|
EDTA, 0.1 mM stabilizes | Rattus norvegicus |
glycerol, 25% stabilizes | Rattus norvegicus |
Tween 80, 0.05% stabilizes | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rattus norvegicus | |
0.058 | - |
2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
59000 | - |
4 * 59000, SDS-PAGE | Rattus norvegicus |
260000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
purified enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, 0.05% Tween 80, 0.1 mM EDTA, 25% glycerol, 5 mM sodium phosphate buffer, pH 7.5, 3 months without loss of activity | Rattus norvegicus |
4°C, 5 h, without stabilizing agents, almost complete loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + 2-amino-4-hydroxy-6-methyltetrahydropterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 59000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
5 h, without stabilizing agent, complete loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | - |
Rattus norvegicus |