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Literature summary for 1.14.16.2 extracted from

  • Okuno, S.; Fujisawa, H.
    Purification and some properties of tyrosine 3-monooxygenase from rat adrenal (1982), Eur. J. Biochem., 122, 49-55.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Dextran sulfate activates the enzyme in the crude extract, less effective with the purified enzyme Rattus norvegicus
heparin activates the enzyme in the crude extract, less effective with the purified enzyme Rattus norvegicus
phosphatidyl-L-serine no stimulation Rattus norvegicus
phosphatidylinositol no stimulation Rattus norvegicus
Phospholipid no stimulation Rattus norvegicus

General Stability

General Stability Organism
EDTA, 0.1 mM stabilizes Rattus norvegicus
glycerol, 25% stabilizes Rattus norvegicus
Tween 80, 0.05% stabilizes Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Rattus norvegicus
0.058
-
2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
Rattus norvegicus
59000
-
4 * 59000, SDS-PAGE Rattus norvegicus
260000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
purified enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, 0.05% Tween 80, 0.1 mM EDTA, 25% glycerol, 5 mM sodium phosphate buffer, pH 7.5, 3 months without loss of activity Rattus norvegicus
4°C, 5 h, without stabilizing agents, almost complete loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + 2-amino-4-hydroxy-6-methyltetrahydropterin + O2
-
Rattus norvegicus ?
-
?
L-tyrosine + tetrahydrobiopterin + O2
-
Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?

Subunits

Subunits Comment Organism
tetramer 4 * 59000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
5 h, without stabilizing agent, complete loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5
-
Rattus norvegicus