General Stability | Organism |
---|---|
EDTA, 0.1 mM stabilizes | Rattus norvegicus |
glycerol, 25% stabilizes | Rattus norvegicus |
Tween 80, 0.05% stabilizes | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Catecholamines | - |
Rattus norvegicus | |
dopamine | - |
Rattus norvegicus | |
epinephrine | - |
Rattus norvegicus | |
norepinephrine | - |
Rattus norvegicus | |
O2 | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
4 * 59000, SDS-PAGE | Rattus norvegicus |
260000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 | Rattus norvegicus | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | ? | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | Rattus norvegicus | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal medulla | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1604 | - |
purified enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, 0.05% Tween 80, 1 mM EDTA, 25% glycerol, 3 months | Rattus norvegicus |
4°C, 24 h, 0.05% Tween 80, 1 mM EDTA, 25% glycerol, 68% loss of activity | Rattus norvegicus |
4°C, 5 h, without stabilizing agents, almost complete loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | Rattus norvegicus | ? | - |
? | |
L-tyrosine + 2-amino-4-hydroxy-6,7-dimethyltetrahydropterin + O2 | synthetic pterin as electron donor | Rattus norvegicus | ? | - |
? | |
L-tyrosine + 2-amino-4-hydroxy-6-methyltetrahydropterin + O2 | synthetic pterin as electron donor | Rattus norvegicus | ? | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? | |
L-tyrosine + tetrahydrobiopterin + O2 | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 59000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile is affected by a variety of conditions: enzymatic phosphorylation by cAMP-dependent protein kinase, calmodulin-dependent protein kinase II and presence of polyanions | Rattus norvegicus |
5.4 | 5.5 | crude extract | Rattus norvegicus |
6 | 7.5 | purified enzyme | Rattus norvegicus |